关键词: 白细胞生成, 环磷酰胺, X射线, 生物反应调节剂

Abstract: Abstract:Objective To observe the leukogenic effect of tremella fuciformis spores polysaccharide (TSP) on leukopenia mice induced by cyclphohamide(CTX) and X-rays. Mice were randomly divided into six groups:normal control group (untreated normal animals); negative control group;positive control group;small,middle,large doses TSP groups.The mice of each group (except normal control group) were administered with CTX（100 mg•kg^-1）　 intraperitoneally or exposed to X-rays to result in leucopenia.In the normal and negative control groups,each animal was administered with saline; in TSP groups,mice were administered with TSP 25,50,100 mg•kg^-1 through caudal vein every day for 15 d; in positive control group,each animal was administered with matrine (200 mg•kg^-1) through caudal vein every day for 15 d.The numbers of peripheral leukocytes of all animals were counted before CTX treatment or exposure to X-rays and 3，6,9,12 and 15 d after treatment.The number of bone marrow karyotes of the mice was examined.Results Compared with normal control group,the leucocytes and the number of bone marrow karyotes of mice were remarkably decreased in the other groups after treated with CTX or exposure to X-rays (P<0.05),but leucocytes and the number of bone marrow karyotes were increased in TSP 50 and 100 mg•kg^-1 groups compared with negative control group (P<0.05).Conclusion TSP has the effect of the leukocytopoiesis promoting in the leukopenia mice induced by CTX and X-rays.

Key words: leukocytopoiesis, cyclphohamide, X-ray, biological response modifier