Abstract:

Objective
To investigate the genetic association between TLR4 gene in chromosome 9q33 and obesity and lung function in Han population from North China.Methods Genomic DNA was isolated from the saliva samples.A single nucleotide polymorphism,rs2149356 (TspEⅠsite) present in the TLR4 gene,was detected using PCR-based restriction fragment length polymorphism(PCR-RFLP) analysis among 506 Chinese Han population.The Hardy-Weinberg equilibrium for genotypic distribution was estimated by the goodness-of-fit χ2 test.The analysis of forced vital capacity (FVC) and the frequencies of alleles and genotypes of TLR4 gene and the relationship between allelic frequencies and obesity and lung function were statistically computed.Results The result of  t test did not show significant difference of FVC between obesity group and control(P>0.05).The  result of goodness-of-fit χ2 test showed that the genotypic distributions of rs2149356 were not deviated from Hardy-Weinberg equilibrium in various groups (P>0.05).The relevance analysis on allelic gene showed that there were no significant differences in the distributions of the frequencies  of allelic A and allelic C between control and obesity groups (P>0.05).The result of χ2 test did not show allelic association and genotypic association for rs2149356 between obesity and FVC (P>0.05).The  result of goodness-of-fit χ2 test showed that the genotypic distributions of rs2149356 were not deviated from Hardy-Weinberg equilibrium in FVC variant groups(P>0.05),too.Conclusion rs2149356 locus may not be associated with obesity and lung function,but the correlation between the other SNPs locus at TLR4 gene and obesity and lung function could not be ruled out.

Key words: obesity;lung function;single nucleotide polymorphism;rs2149356