肿瘤相关巨噬细胞来源IL-6通过上调肿瘤细胞中LIF表达对口腔鳞状细胞癌Cal27细胞侵袭和迁移的促进作用

doi:10.13481/j.1671-587X.20220414

Abstract

Abstract: Background

To investigate the effects of tumor associated macrophages（TAMs）-derived interleukin-6 （IL-6） on the leukemia inhibitory factor （LIF） expression in oral squamous cell carcinoma （OSCC） Cal27 cells and the invasion and migration of OSCC Cal27 cells，and to clarify their possible mechanisms.

Methods

The human OSCC Cal27 cells and mouse peritoneal macrophages（Raw264.7 cells ）were cultured in vitro. The Raw264.7 cells were divided into control and experimental groups. The cells in control group were incubated with DMEM， and the cells in experimental group were incubated with Cal27 conditioned medium （Cal27-CM） to obtain the TAMs. The expression levels of IL-6 mRNA and IL-6 levels in the cells in two groups were detected by real-time fluorescence quantitative PCR （RT-qPCR） and enzyme-linked immunosorbent assay （ELISA） methods. The Cal27 cells were divided into control group and experimental group （5 μg·L^－1 Tocilizumab）.The Cal27 cells in control group were directly treated with TAMs conditioned medium， and the Cal27 cells in experimental group were treated with interleukin 6 receptor （IL-6R） inhibitor Tocilizumab in advance and then treated with TAMs conditioned medium. Transwell chamber experiment was used to detect the migration and invasion abilities of Cal27 cells in two groups. Cell scratch assay was used to detect the scratch healing rates of Cal27 cells in two groups. RT-qPCR and Western blotting methods were used to detect the expression of levels LIF mRNA and protein in Cal27 cells in two groups.

Results

The results of RT-qPCR and ELISA showed that the mRNA expression level of IL-6 and the IL-6 level in the TAMs in experimental group were significantly higher than those in the RAW264.7 cells in control group （P<0.01）. The results of Transwell chamber experiment showed that compared with control group， the number of migration and invasion cells in experimental group was significantly reduced （P<0.01）. The results of cell scratch experiment showed that compared with control group， the scratch healing rate of the Cal27 cells in experimental group was significantly decreased （P<0.01）. The RT-qPCR and Western blotting results showed that compared with control group， the expression levels of LIF mRNA and protein in the Cal27 cells in experimental group were decreased down-regulated （P<0.01）.

Conclusion

TAMs-derived IL-6 promotes the invasion and migration of OSCC Cal27 cells by up-regulating the expression of LIF in tumor cells.

Key words: Leukemia inhibitory factor, Oral squamous cell carcinoma, Tumor associated macrophages, Interleukin-6

CLC Number:

R739.8

Weibo LI,Juan CAO,Xiu GUO,Chunling DONG,Bo LI. Promotion effect of tumor associated macrophages-derived IL-6 on invasion and migration of oral squamous cell carcinoma Cal27 cells by upregulating LIF expression in tumor cells[J].Journal of Jilin University(Medicine Edition), 2022, 48(4): 946-953.

Figures/Tables 7

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References 35

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Promotion effect of tumor associated macrophages-derived IL-6 on invasion and migration of oral squamous cell carcinoma Cal27 cells by upregulating LIF expression in tumor cells

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