沉默TRPV2基因和大麻二酚对口腔鳞状细胞癌CAL-27细胞生物学行为的影响

doi:10.13481/j.1671-587X.20260115

Abstract

Abstract:

Objective To discuss the effects of transient receptor potential vanilloid channel 2 （TRPV2） on the proliferation， migration， and invasion of oral squamous cell carcinoma （OSCC） cells and the effect of cannabidiol （CBD） on the biological behaviors of OSCC cells through the TRPV2 channel， and to clarify its related anti-tumor mechanism. Methods The siRNA fragments were transfected into the CAL-27 cells using the liposome method， and the cells were divided into si-NC group （transfected with non-related si-NC） and si-TRPV2 group （transfected with specific siRNA silencing TRPV2 gene）. The CAL-27 cells were cultured with different doses of CBD and divided into 0， 10， 20， and 40 μmol·L^-1 CBD groups. Real-time fluorescence quantitative PCR （RT-qPCR） method and Western blotting method were used to detect the expression levels of TRPV2 mRNA and protein in the cells in various groups； cell counting kit-8 （CCK-8） method， cell scratch healing assay， and Transwell chamber assay were used to detect the proliferation activity， scratch healing rate， and number of invasion CAL-27 cells in various groups， respectively. Results The RT-qPCR method and Western blotting method results showed that the expression levels of TRPV2 mRNA and protein in the CAL-27 cells in si-NC group were higher than those in si-TRPV2 group （P<0.01）. The CCK-8 assay results showed that compared with si-NC group， the proliferation activities of the cells in si-TRPV2 group at 48 and 72 h after transfection were significantly decreased （P<0.01）. The cell scratch healing assay results showed that compared with si-NC group， the scratch healing rate of the CAL-27 cells in si-TRPV2 group at 24 h was significantly decreased （P<0.01）. The Transwell chamber assay results showed that compared with si-NC group， the number of invasion cells in si-TRPV2 group was significantly decreased （P<0.01）. The RT-qPCR method and Western blotting method results showed that compared with 0 μmol·L^-1 CBD group， the expression levels of TRPV2 mRNA and protein in the CAL-27 cells in 10， 20， and 40 μmol·L^-1 CBD groups were decreased （P<0.05 or P<0.01）. The CCK-8 assay results showed that compared with 0 μmol·L^-1 CBD group， the cell survival rates of the cells in 20 and 40 μmol·L^-1 CBD groups at 48， 72， and 96 h after drug culture were significantly decreased （P<0.01）. The cell scratch healing assay results showed that after CBD culture for 12 and 24 h， compared with 0 μmol·L^-1 CBD group， the scratch healing rates of the CAL-27 cells in 10， 20， and 40 μmol·L^-1 CBD groups were significantly decreased （P<0.01）. The Transwell chamber assay results showed that compared with 0 μmol·L^-1 CBD group， the number of invasion cells in 40 μmol·L^-1 CBD group was significantly decreased （P<0.01）. Conclusion Silencing the TRPV2 gene and CBD treatment can both inhibit the proliferation， migration， and invasion abilities of CAL-27 cells， and CBD can reduce the expression of TRPV2 gene and protein in CAL-27 cells.

Key words: Transient receptor potential vanilloid channel 2, Oral squamous cell carcinoma, Cannabidiol, Cell migration, Cell invasion

CLC Number:

R739.8

Yunshan DING,Haitao DAI,Min CHEN,Xiaohui HAO,Xiao ZHOU,Nan WU. Effect of silencing TRPV2 gene and cannabidiol on biological behaviors of oral squamous cell carcinoma CAL-27 cells[J].Journal of Jilin University(Medicine Edition), 2026, 52(1): 143-151.

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References 27

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Group	Survival rate
Group	（t/h） 24	48	72	96
CBD （μmol·L^-1）
0	100.00±11.84	100.00±6.61	100.00±7.54	100.00±12.08
10	96.98±7.41	108.70±17.11	118.50±4.76^*	100.80±15.30
20	35.35±1.91^*	17.06±15.87^*	4.36±0.19^*	3.98±0.64^*
40	16.95±0.94^*	10.29±5.07^*	3.99±1.26^*	2.84±1.65^*

Effect of silencing TRPV2 gene and cannabidiol on biological behaviors of oral squamous cell carcinoma CAL-27 cells

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