Abstract: Abstract:Objective
To explore the effect of schisandrin B(Sch B) on expression of vascular endothelial growth factor (VEGF) in glioma SHG-44 cells and its inhibitory effect on cell growth and to explore the possible mechanism.Methods Glioma SHG-44 cells were separated to control and 3 treatment groups,and then treated with 0,50,100 and 200 mg•L^-1  Sch B for 24-72 h.MTT assay was used to evaluate the proliferation of glioma SHG-44 cells.The levels of VEGF protein expression after treated with Sch B were measured by both ELISA and immuncytochemistry.Results Compared with control group ,the proliferation activity of glioma SHG-44 cells was slightly increased after  treated with 200 mg•L^-1 Sch B for 24 h(P<0.05);the proliferation abilities of the SHG-44 cells  were obviously decreased after treated with  50,100 and 200 mg•L^-1  Sch B for 48 h or 72 h;the VEGF protein level in the supernatant of cultured cells after treated with Sch B for 72 h was significantly decreased (P<0.01).After the glioma  SHG-44 cells were treated with Sch B for 72 h,the positive expression rates of VEGF protein in 50,100 and 200 mg•L^-1 groups were markedly reduced compared with control group (P<0.01).Conclusion Sch B can inhibit the expression of VEGF protein and growth of glioma SHG-44 cells,it suggests that Sch B may exert anti-glioma effect by inhibiting angiogenesis in glioma.

Key words: schisandrin B, glioma, vascular endothelial growth factor, cell proliferation