MARCH1通过PI3K/AKT信号通路对人胃癌细胞迁移和侵袭的促进作用

doi:10.13481/j.1671-587X.20210214

Abstract

Abstract: Objective

To investigate the effects of membrane associated RING-CH 1 （MARCH1） on the migration and invasion of gastric cancer cells， and to clarify its possible molecular mechanism.

Methods

A total of 20 cases of gastric cancer tissue and adjacent tissue obtained from gastrectomy were collected， and the expression levels of MARCH1 mRNA and protein in the different tissues were detected by Real-time fluorescence quantitative polymerase chain reaction （RT-qPCR） method and Western blotting method. The expression levels of MARCH1 mRNA in the human gastric mucosal normal epithelial cells GES-1 and the human gastric cancer cells BGC-823， BGC-803， AGS and SGC-7901 were detected by RT-qPCR method. The SGC-7901 cells in logarithmic growth phase were divided into siNC group（transfected with siNC）， siMARCH1-1 group（transfected with siMARCH1-1） and siMARCH1-2 group（transfected with siMARCH1-2）. RT-qPCR method and Western blotting method were used to detect the expression levels of MARCH1 mRNA and protein in the cells in various groups. CCK-8 method was used to detect the cell proliferation activities in various groups； cell scratch test was used to detect the scratch healing rates of the cells in various groups； Transwell chamber test was used to detect the cell invasion abilities in various groups. The expression levels of phosphorylated phosphatidylinositol 3-kinase （p-PI3K）， phosphorylated protein kinase B（p-AKT） and protein kinase B （AKT） proteins in various groups were detected by Western blotting method.

Results

Compared with the adjacent tissue， the expression levels of MARCH1 mRNA and protein in the gastric cancer tissue were increased （P<0.05）. Compared with the human gastric mucosal normal epithelial cells GES-1， the expression levels of MARCH1 mRNA in human gastric cancer cells BGC-823， BGC-803， AGS， and SGC-7901 were all increased （P<0.05 or P<0.01）. Compared with siNC group， the expression levels of MARCH1 mRNA and protein in siMARCH1-1 group and siMARCH1-2 group were significantly reduced （P<0.01）. Compared with siNC group， the cell proliferation activities in siMARCH1-1 group and siMARCH1-2 group had no significant differences（P>0.05）； the cell scratch healing rates and the number of invasion cells in siMARCH1-1 group and siMARCH1-2 group were significantly reduced （P<0.01 ）. The expression levels of p-PI3K and p-AKT proteins in siMARCH1-1 group and siMARCH1-2 group were significantly reduced compared with siNC group （P<0.01）， and the expression levels of AKT protein had no significant differences（P>0.05）.

Conclusion

MARCH1 can promote the migration and invasion of gastric cancer cells， and its mechanism may be related to the regulation of PI3K/AKT signaling pathway.

Key words: stomach neoplasm, membrane associated RING-CH 1, cell migration, cell invasion, phosphatidylinositol 3-kinase, protein kinase B

CLC Number:

R735.2

Nuan WANG,Lijuan YANG,Juanjuan DAI,Aili WANG,Yan WU,Chengxia LIU. Promotion effects of MARCH1 on migration and invasion of human gastric cancer cells through PI3K/AKT signaling pathway[J].Journal of Jilin University(Medicine Edition), 2021, 47(2): 352-359.

Figures/Tables 7

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Promotion effects of MARCH1 on migration and invasion of human gastric cancer cells through PI3K/AKT signaling pathway

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