重组人IL-17A在胃癌组织中的表达及其对胃癌BGC-823细胞增殖、侵袭、迁移和凋亡的影响

doi:10.13481/j.1671-587X.20210622

Abstract

Abstract: Objective

To detect the expression of human interleukin-17A （IL-17A） recombinant protein in the human gastric cancer tissue and its effects on the proliferation， invasion， migration and apoptosis of gastric cancer cells， and to explore their mechanisms.

Methods

Thirty cases of postoperative paraffin specimens of the patients with gastric cancer，20 cases of intestinal metaplasia specimens， and 10 cases of normal gastric tissue were collected. The positive expression rates of IL-17A and interleukin-17 receptor A （IL-17RA） in different kinds of tissues were detected by immunohistochemical staining.The correlation between the expressions of IL-17A and IL-17RA was analyzed by Pearson correlation analysis.The BGC-823 cells in logarithmic growth phase were divided into control group and different concentrations （50，100， and 200 μg·L^－1） of IL-17A groups. The proliferation activities of the gastric cancer BGC-823 cells in various groups were detected by MTT method. The numbers of invasion cells of the gastric cancer BGC-823 cells in various groups were detected by Transwell test. The migration rates of the gastric cancer BGC-823 cells in various groups were detected by scratch test， and the expression levels of vascular endothelial growth factor （VEGF），matrix metalloproteinase-9 （MMP-9），B cell lymphoma-2 （Bcl-2）， and Bcl-2 associated X protein （Bax） in the gastric cancer BGC-823 cells in various groups were detected by Western blotting method.

Results

The positive expression rates of IL-17A and IL-17RA in the intestinal metaplasia and gastric cancer tissue were significantly higher than those in normal gastric tissue（P<0.01）. There was positive correlation between IL-17A expression and IL-17RA expression in the gastric cancer tissue（r=0.891，P<0.01）. Compared with control group，the proliferation activities of the gastric cancer BGC-823 cells in 100 and 200 μg·L^－1 IL-17A groups were significantly increased after treated with IL-17A for 48 and 72 h （P<0.05）. Compared with control group， the apoptotic rates of gastric cancer BGC-823 cells in 100 and 200 μg·L^－1 IL-17A groups were decreased significantly （P<0.01）， the numbers of invasion cells and migration rates were increased （P<0.05）.The Western blotting method results showed that compared with control group， the expression levels of VEGF， MMP-9 and Bcl-2 proteins in the gastric cancer BGC-823 cells in 200 μg·L^－1 IL-17A group were increased （P<0.05）， and the expression level of Bax protein was decreased（P<0.05）.

Conclusion

The recombinant human IL-17A can promote the proliferation， migration， and invasion of the gastric cancer BGC-823 cells and inhibits the apoptosis，and its mechanism may be related to the promotion of expressions of invasion-related proteins VEGF， MMP-9， and the inhibition of apoptosis-related proteins Bcl-2 and Bax.

Key words: interleukin-17A, interleukin-17 receptor A, stomach neoplasms, apoptosis, cell migration

CLC Number:

R735.2

Runhong MU,Yijiu AI,Yupeng LI,Rui LIN,Siping YE,Fang MA,Xiao GUO. Expression of recombinant human IL-17A in gastric cancer tissue and its effects on proliferation, invasion, migration and apoptosis of gastric cancer BGC-823 cells[J].Journal of Jilin University(Medicine Edition), 2021, 47(6): 1510-1517.

Figures/Tables 8

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Group	Proliferation activity
	(t/h) 24	48	72
Control IL-17A(μg·L^－1) 50 100 200	0.233±0.018	0.404±0.029	0.451±0.017 0.506±0.025 0.558±0.024^* 0.672±0.032^*
	0.239±0.021	0.487±0.023
	0.280±0.026	0.512±0.031^*
	0.315±0.032	0.647±0.027^*

Group	VEGF	MMP-9	Bcl-2	Bax
Control	0.720±0.026	0.410±0.018	0.490±0.025	0.640±0.031
200 μg·L^－1 IL-17A	1.110±0.054^*	0.870±0.041^*	0.750±0.029^*	0.820±0.034^*

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Expression of recombinant human IL-17A in gastric cancer tissue and its effects on proliferation, invasion, migration and apoptosis of gastric cancer BGC-823 cells

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