吉林大学学报(工学版) ›› 2017, Vol. 47 ›› Issue (1): 332-336.doi: 10.13229/j.cnki.jdxbgxb201701048

• Orginal Article • Previous Articles    

Neuroprotective effects of soy isoflavones on PC12 cells based on antioxidation

XU Meng-lei1, GAO Yu2, LIU Jing-bo1, XIONG Jin-feng3, ZHAO Song-ning1, HUANG Yan-jun1   

  1. 1.Laboratory of Nutrition and Functional Food, Jilin University, Changchun 130062, China;
    2.College of Agriculture, Jilin Agricultural University, Changchun 130018, China;
    3.Changchun Institute of Biological Products Co., Ltd., Changchun 130012, China
  • Received:2016-01-11 Online:2017-01-20 Published:2017-01-20

RICH HTML

0   

PDF (PC)

348

Abstract: Experiment results show that when PC12 cells were exposed to 20.00 μg/mL genistein (GEN), daidzein (DAI) or their combination (GEN-DAI), the cells' viability rates, mophologies, mitochondrial membrane potential, superoxide dismutase activities and inhibition rates were not changed. However, different changes of PC12 cells were observed under different treatments. The reactive oxygen species level, the relative fluorescence intensity and malondialdehyde of PC12 cells were increased by GEN. The leakage rate of lactic dehydrogenase was decreased in DAI and in GEN-DAI combination treatments, the glutathione contents were increased to (297.27±9.47) μmol/(g·prot) and (269.90±00) μmol/(g·prot) respectively. The acetylcholine content in culture medium and metabolism of cholinergic system was increased in GEN-DAI treatment. GEN-DAI combination treatment can more significantly increase the activity of the antioxidant system of PC12 cells than GEN and DAI treatments.

Key words: food processing technology, PC12 cells, soy isoflavones, antioxidant, neuroprotective effect, genistein, daidzein

CLC Number: 

  • TS201
[1] Xu M L, Liu J, Zhu C, et al. Interactions between soy isoflavones and other bioactive compounds: a review of their potentially beneficial health effects[J]. Phytochem Rev, 2015, 14(3):459-467.
[2] Murphy P A, Barua K, Hauck C C. Solvent extraction selection in the determination of isoflavones in soy foods[J]. Journal of Chromatography B, 2002, 777(1/2):129-138.
[3] Vacek J, Klejdus B, Lojkova L, et al. Current trends in isolation, separation, determination and identification of isoflavones: a review[J]. J Sep Sci, 2008, 31(11):2054-2067.
[4] Donzelli A, Braida D, Finardi A, et al. Neuroprotective effects of genistein in Mongolian gerbils: estrogen receptor-β involvement[J]. J Pharmacol Sci, 2010, 114(2):158-167.
[5] Valles S L, Dolz-Gaiton P, Gambini J, et al. Estradiol or genistein prevent Alzheimer's disease-associated inflammation correlating with an increase PPARc expression in cultured astrocytes[J]. Brain Res, 2010, 1312:138-144.
[6] Kim D K, Chancellor M B. Is estrogen for urinary incontinence good or bad?[J].Rev Urol, 2006,8(2):91-92.
[7] Allred C D, Allred K F, Ju Y H, et al. Soy processing influences growth of estrogen-dependent breast cancer tumors[J]. Carcinogenesis, 2004, 25(9):1649-1657.
[8] Goldstein D S, Sullivan P, Cooney A, et al. Vesicular uptake blockade generates the toxic dopamine metabolite 3, 4-dihydroxyphenylacetaldehyde in PC12 cells: relevance to the pathogenesis of Parkinson's disease[J]. J Neurochem, 2012, 123(6):932-943.
[9] Liu J, Chen Z, He J, et al. Anti-oxidative and anti-apoptosis effects of egg white peptide, Trp-Asn-Trp-Ala-Asp, against H 2 O 2 -induced oxidative stress in human embryonic kidney 293 cells[J]. Food Funct, 2014, 5(12):3179-3188.
[10] 陈志飞. 蛋清源抗氧化肽对HEK293细胞氧化应激损伤的抑制作用及机制研究[D]. 长春:吉林大学军需科技学院, 2014.
Chen Zhi-fei. The aiti-oxidative effects and mecanism of antioxidant peptides from egg hite against oxidantive stress injury in human embryonic kidney 293 cells[D]. Changchun: College of Quartermaster Technology, Jilin University, 2014.
[11] 赵凡桂, 王文君, 周文江, 等. 更年春方对切除卵巢大鼠学习记忆能力和海马胆碱能系统的影响[J]. 中国中西医结合杂志, 2008, 28(3):234-237.
Zhao Fan-gui, Wang Wen-jun, Zhou Wen-jiang, et al. Effect of gengianchun recipe on learning memory function and hippocampal cholinergic system in ovariectomized rats[J]. Chinese Journal of Integrated Traditional and Western Medicine, 2008, 28(3):234-237.
[12] 蔡标, 汪远金, 王艳, 等. 大豆异黄酮对阿尔茨海默病大鼠乙酰胆碱代谢的影响[J]. 安徽中医药大学学报, 2013, 32(1):57-60.
Cai Biao, Wang Yuan-jin, Wang Yan,et al. Effect of soybean isoflavone on acetylcholine metabolism in rats with Alzheimer's disease[J]. Journal of Anhui University of Chinese Medicine, 2013, 32(1):57-60.
[1] ZHANG Tie-hua, JIANG Nan, LIU Di-ru, HAO Jin-feng, MAO Chun-ling, GUO Ming-ruo. Preparation of whey protein based fat replacer and application in low fat milk [J]. 吉林大学学报(工学版), 2015, 45(3): 1024-1028.
[2] ZHAO Mou-ming,LONG Zhao,ZHAO Qiang-zhong,XU Ju-cai. Effects of protein content and composition on the physicochemical properties of whipping cream [J]. 吉林大学学报(工学版), 2014, 44(5): 1531-1536.
[3] YUAN Yuan,ZHANG Huan-jie, MIAO Yu-tian,WU Si-jia, LIN Song-yi,LIU Jing-bo. Contribution of the frying condition of potato slices to the formation of acrylamide [J]. 吉林大学学报(工学版), 2014, 44(5): 1525-1530.
[4] JIN Sheng-lang, WANG Ying, YIN Yong-guang. Effect of high intensity pulsed electric field pretreatment on elasticity of starch-myofibril protein mixed gelatin [J]. 吉林大学学报(工学版), 2014, 44(2): 573-578.
[5] WANG Li-min, XIAO Zhi-gang, LIU Yu-xin, LI Jie, SUN Xu, SHEN Xun-ye, LI Jia-dong. Optimization of extrusion process parameters of nutritious rice rich in chestnut by response surface method [J]. 吉林大学学报(工学版), 2013, 43(02): 550-556.
[6] WANG Xin, HOU Ju-min, CONG Xian-ling. Composition analysis of the whey fermentation product with the antioxidant activity [J]. 吉林大学学报(工学版), 2012, 42(增刊1): 444-448.
[7] LIU Jing-bo, CHANG Hao, WANG Er-lei, GONG Xin-tong, JIANG Yi-qun. Phosphatidylcholine of egg yolk lecithin detection using high performance liquid chromatography(HPLC) [J]. 吉林大学学报(工学版), 2012, 42(增刊1): 449-453.
[8] LIU Jing-bo, TAO Xu, JIANG Wei, DING Long, WANG Er-lei. Technology optimization of Sialic acid isolation from hen eggshell membrane by quadric regression orthogonal rotary tests [J]. , 2012, 42(04): 1071-1076.
[9] SUN Zhong-lei, SUN Yong-hai, LI Yu, LIU Jing-jing. Establishment and validation of the food fracture mechanical model based on the bionic indenter [J]. 吉林大学学报(工学版), 2012, 42(02): 510-514.
[10] ZHUANG Hong, CHEN Le-qun, ZHANG Ting, ZHU Yuan-yuan, TANG Ning, YUAN Yuan. Property of compound protective agents on ferrous iron of heme-iron enriched polypeptide [J]. 吉林大学学报(工学版), 2012, 42(01): 256-260.
[11] LIU Jing-bo, WANG Fei, WANG Cui-na, LIU Jun, WANG Zuo-zhao, WANG Er-lei, ZHANG Yan, LIN Song-yi. Optimization of preparation for anticoagulant peptide from egg white powder by Alcalase [J]. 吉林大学学报(工学版), 2012, 42(01): 250-255.
[12] WANG Xin-wei, LIU Huan, MA Zhong-su. The properties of chitosan/corn starch/gelatin/carrot edible films [J]. 吉林大学学报(工学版), 2011, 41(03): 887-892.
[13] ZHUANG Hong,ZHU Yuan-yuan,ZHANG Ting,CHEN Le-qun,LIU Jing-bo. Enzymatic preparation and protection technology of heme [J]. 吉林大学学报(工学版), 2011, 41(03): 869-875.
[14] XU Cai-na,YIN Yong-guang,WANG Er-lei,LIU Jing-bo,LIN Song-yi,ZHANG Gang. Optimized process of extracting phosvitin from hen egg yolk based on genetic algorithm [J]. 吉林大学学报(工学版), 2011, 41(03): 876-881.
[15] ZHANG Tie-hua,WANG Shao-jun,LIU Di-ru,YUAN Yuan,YU Ya-li,YIN Yong-guang. Optimization of exopolysaccharide extraction process from Tibetan spiritual mushroom by pulsed electric fields [J]. 吉林大学学报(工学版), 2011, 41(03): 882-886.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright © Journal of Jilin University(Engineering and Technology Edition), All Rights Reserved.
Tel: +86-431-85095297　Fax: +86-431-85094074　E-mail: xbgxb@jlu.edu.cn
Powered by Beijing Magtech Co. Ltd