吉林大学学报(工学版) ›› 2004, Vol. ›› Issue (4): 578-581.

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Plytase producing property of high-yield mutant of cell-surface phytase yeast

LIANG Yanlong, ZHAO Shoujing, JIANG Lei, ZHAN Wenzong   

  1. College of Biological and Agricultural Engineering, Jilin University, Changchun 130022, China
  • Received:2003-10-26 Online:2004-10-01

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Abstract: The behavior of a high-yield mutant WZ-4 bacterium of the cell-surface phytase yeast was studied. The results indicate that the optimum pH value for the yeast is 5, the optimum temperature is 50 ℃, and the Km value for the Na-phytate is 0.666 mmol/L. Besides, the phytase production of WZ-4 bacterium is controlled by the phosphorus concentration in the culture medium. The maximum production of phytase occurrs in the culture medium containing 0.5 mg phosphorus per 100mL. When the concentration of phosphorus in the medium is more than 10 mg phosphorus per 100mL, the phytase production is completely repressed.

Key words: biochemistry engineering, enzyme, cell-surface phytase, yeast, repression

CLC Number: 

  • O556.9
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