吉林大学学报(工学版) ›› 2012, Vol. 42 ›› Issue (增刊1): 466-469.

Previous Articles     Next Articles

Purification of anticoagulant peptides derived from egg white powder

LIU Jing-bo1, WANG Fei1, ZHANG Yan1, WANG Er-lei1, WANG Zuo-zhao1, JIANG Yi-qun2   

  1. 1. Laboratory of Nutrition Functional Food, Jilin University, Changchun 130062, China;
    2. College of Life Science, Jilin University, Changchun 130021, China
  • Received:2011-08-09 Online:2012-09-01 Published:2012-09-01

Abstract: The anticoagulant peptides derived from egg white powder were hydrolysised by Alcalase and then purified by SephadexG-50 and semi-preparative reversed phaseliquid chromatography (RPLC). High activity fraction was selected after rotary evaporation and freeze drying. The anticoagulant activity was determined by micro plate reader. The results showed that the No.4 fraction of eluted by SephadexG-50 at time 35~40 min had high anticoagulant activities which were 84.74%. Then, this fraction was purified by semi-preparative reversed phaseliquid chromatography in which it separated to 12 peaks. Peak Ⅶ was eluted at 17.275~19.542 min. The highest anticoagulant activities of fractions was peak Ⅶ which inhibition rate reached up to 71.27%.

Key words: basic discip line of food science and technology, anticoagulant peptide, gel chromatography, semi-preparative high performance liquid chromatograph

CLC Number: 

  • TS201.4
[1] 新华社. 心脑血管病成为我国发病率死亡率最高疾病..http:[C]//www.ebiotrade.com/newsf/2009-3/200932393459.html.

[2] Magalhaes A , Magalhaes H P, Richardson M,et al. Purification and properties of a coagulant thrombin-like enzyme from the venom of Bothrops leucurus[J]. Comparative Biochemistry and Physiology, 2007, 146(4):565-575.

[3] Kong Yi, Huo Jian-li, Xu Wen, et al. A novel anti-platelet aggregation tripeptide from Agkistrodon acutus venom: Isolation and characterization[J]. Toxicon, 2009, 54(2):103-109.

[4] Sanchez E E, Rodriguez-Acosta A, Palomar R, et al. Colombistatin: a disintegrin isolated from the venom of the South American snake (Bothrops colombiensis) that effectively inhibits platelet aggregation and SK-Mel-28 cell adhesion[J]. Archives of Toxicology, 2009, 83(3):271-279.

[5] Barbouche R, Marrakchi N, Mansuelle P, et al. Novel anti-platelet aggregation polypeptides from Vipera lebetina venom: Isolation and characterization[J]. Febs Letters, 1996, 392(1):6-10.

[6] Osipov A V, Filkin S Y, Makarova Y V, et al. A new type of thrombin inhibitor, noncytotoxic phospholipase A2, from the Naja haje cobra venom[J]. Toxicon, 2010, 55(2-3):186-194.

[7] Giron M E, Estrella A, Sanchez E E, et al. Purification and characterization of a metalloproteinase, Porthidin-1, from the venom of Lansberg's hog-nosed pitvipers (Porthidium lansbergii hutmanni)[J]. Toxicon, 2011, 56(4):608-618.

[8] Won-Kyo J, Se-Kwon K. Isolation and characterisation of an anticoagulant oligopeptide from blue mussel, Mytilus edulis[J]. Food Chemistry, 2009, 117(4):687-692.

[9] Gan W Q, Deng L, Yang Chen, et al. An anticoagulant peptide from the human hookworm, Ancylostoma duodenale that inhibits coagulation factors Xa and XIa[J]. Febs Letters, 2009, 583(12):1976-1980.

[10] Vija H, Samel M, Siigur E, et al. Purification, characterization, and cDNA cloning of acidic platelet aggregation inhibiting phospholipases A2 from the snake venom of Vipera lebetina (Levantine viper)[J]. Toxicon , 2009, 54(4):429-439.

[11] Jo H Y, Jung W K, Kim S K, et al. Purification and characterization of a novel anticoagulant peptide from marine echiuroid worm, Urechis unicinctus[J]. Process Biochemistry, 2008, 43(2):179-184.

[12] 田刚,陈代文,余冰,等. 酶解鸡蛋清小肽混合物对小鼠免疫功能的影响[J]. 中国畜牧杂志,2005,41(5):14-17. Tian Gang, Chen Dai-wen, Yu Bing, et al. Effects of mixtures of small peptides from enzymic hydrolysates of egg white on immune functions of mice[J]. Chinese Journal of Animal Science, 2005, 41(5):14-17.

[13] Miguel Marta, Lopez-Fandino Rosina, Ramos Mercedes, et al. Long-term intake of egg white hydrolysate attenuates the development of hypertension in spontaneously hypertensive rats[J]. Life Science, 2006, 78(25):2960-2965.

[14] Yang W G, Wang Z, Xu S Y. A new method for determination of antithrombotic activity of egg white protein hydrolysate by microplate reader[J]. Chinese Chemical Letters, 2007, 18(4):449-451.
[1] LIU Jing-bo, WANG Fei, WANG Cui-na, LIU Jun, WANG Zuo-zhao, WANG Er-lei, ZHANG Yan, LIN Song-yi. Optimization of preparation for anticoagulant peptide from egg white powder by Alcalase [J]. 吉林大学学报(工学版), 2012, 42(01): 250-255.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!