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• 生命科学 • 上一篇    下一篇

新疆察吾呼沟古代居民线粒体DNA序列多态性分析

谢承志1,2, 刘树柏2, 崔银秋1,2, 朱 泓1, 周 慧1,2   

  1. 1. 吉林大学边疆考古中心考古DNA实验室, 长春 130012; 2. 吉林大学生命科学学院大分子实验室, 长春 130021
  • 收稿日期:2004-11-02 修回日期:1900-01-01 出版日期:2005-07-26
  • 通讯作者: 周 慧

Analysis of Mitochondrial DNA from the Ancient Tombs of Chawuhu

XIE Cheng-zhi1,2, LIU Shu-bai2, CUI Yin-qiu1,2 , ZHU Hong1, ZHOU Hui1,2   

  1. 1. Ancient DNA Laboratory, Center of Border Archaeology, Jilin University, Changchun 130012, China; 2. Macromolecular Laboratory, College of Life Science, J ilin University, Changchun 130021, China
  • Received:2004-11-02 Revised:1900-01-01 Online:2005-07-26
  • Contact: ZHOU Hui

摘要: 从距今2 500~3 000年的新疆察吾呼沟墓地的9例古代人骨中成功地提取出古DNA分 子. 用4对套叠引物对线粒体DNA高可变Ⅰ区进行了PCR扩增和测序, 得到9个364 bp的线粒 体序列. 从GenBank搜索其共享序列并与欧洲、 亚洲序列进行系统发育分析, 结果表明, 早在青铜至早期铁器时代, 在我国新疆天山中部地区已经有蒙古人种存在. 察吾呼沟古代 居民应是一个欧洲和东亚人种混合的古代群体.

关键词: 察吾呼, 古DNA, 序列分析, 多态现象

Abstract: The DNA was successfully extracted from the human bone samples excava ted from the ancient tombs of Chawuhu, which has been dated back to 2 500~ 3 000 years ago according to the feature of pottery and a series of 14C data. Part of the mitochondrial hypervariable region Ⅰ(364 bp) was amplifi ed and sequenced with four overlapping primers. In order to search their sharin g sequences and determine their pedigree, each sequence was searched and compare d with the database GenBank. Phylogenetic analysis indicated that there existed the admixture of Euro-Asian lineage in the east of Xinjiang prior to the time o f the Bronze Age.

Key words: Chawuhu, ancient DNA, sequence analysis, polymorphism

中图分类号: 

  • Q987