近红外漫反射光谱法对蛇床子SFE萃取产物的质量监控

近红外漫反射光谱法对蛇床子SFE萃取产物的质量监控

李文良¹, 弥宏², 窦英¹, 陈永红¹, 王友兵³, 任玉林¹

1. 吉林大学化学学院，长春 130021； 2. 吉林省中医中药研究院新药中心试剂室，长春 130021； 3. 白城医学高等专科学校化学教研室，吉林省白城 137000

收稿日期:2005-07-14 修回日期:1900-01-01 出版日期:2006-07-26 发布日期:2006-07-26
通讯作者: 任玉林

Application of Nearinfrared Diffuse Reflectance Spectroscopy in Controlling Extractant of Cnidium Monieri by Supercritical CO2

LI Wenliang¹, MI Hong², DOU Ying¹, CHEN Yonghong¹, WANG Youbing³, REN Yulin¹

1. College of Chemistry, Jilin University, Changchun 130021, China; 2. Pharmaceutical Research Center, Jilin Institute of Traditional Chinese Medicine, Changchun 130021, China; 3. Department of Chemistry, Baicheng Medical College, Baicheng 137000, Jilin Province, China

Received:2005-07-14 Revised:1900-01-01 Online:2006-07-26 Published:2006-07-26
Contact: REN Yulin

摘要/Abstract

摘要： 采用近红外漫反射光谱法对中药蛇床子SFE萃取过程中主要成分蛇床子素和欧前胡素进行快速、无损检测. 以HPLC分析值作参比值，采用偏最小二乘法（PLS）建立近红外光谱信息与各组分含量间的定量校正模型. 讨论了波长范围的选择和主成分数对PLS定量预测蛇床子萃取物中蛇床子素和欧前胡素含量能力的影响，并对预测集样品含量进行预测，结果令人满意.

关键词: 近红外漫反射光谱, 偏最小二乘法, 蛇床子, 超临界CO₂萃取

Abstract: A rapid and nondestructive method using nearinfrared diffuse reflectance spectroscopy was presented to determine the principal components (osthol and imperatorin) of Cnidium monieri  extracted by supercritical carbon dioxide. Partial least squares (PLS) method was utilized to establish the calibration models for describing the relationship between the nearinfrared spectral information and each component with high performance liquid chromatography (HPLC) as a reference method. The effects of the region sele ction of wavelength and the numbers of principal components on the prediction ab ility of PLS were discussed and the concentrations of the samples of the prediction set were predicted, the predicted results were reliable.

Key words: nearinfrared diffuse reflectance spectrum, partial least squares, C nidium monieri, extraction of supercritical carbon dioxide 

中图分类号:

O657.33

李文良, 弥宏, 窦英, 陈永红, 王友兵, 任玉林. 近红外漫反射光谱法对蛇床子SFE萃取产物的质量监控[J]. J4, 2006, 44(04): 645-648.

LI Wenliang, MI Hong, DOU Ying, CHEN Yonghong, WANG Youbing, REN Yulin. Application of Nearinfrared Diffuse Reflectance Spectroscopy in Controlling Extractant of Cnidium Monieri by Supercritical CO2[J]. J4, 2006, 44(04): 645-648.

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