醇 碱 提 取 法 提 取 黄 芪 多 糖

醇碱提取法提取黄芪多糖

田洛¹，宣依昉¹，范荣军¹，姜云垒²，邢茗¹，刘立东¹，陈霞¹

1.　吉林大学生命科学学院，长春 130021； 2.　吉林农业大学动物科技学院，长春 130018

收稿日期:2005-12-30 修回日期:1900-01-01 出版日期:2006-07-26 发布日期:2006-07-26
通讯作者: 陈霞

Ethanolalkali Extraction Method of Astragalus Polysaccharides

TIAN Luo¹, XUAN Yifang¹, FAN Rongjun¹, JIANG Yunlei², XING Ming¹, LIU Lidong¹, CHEN Xia¹

1.　College of Life Science, Jilin University, Changchun 130021, China; 2.　College of Animal Technology, Jilin Agricultural University, Changchun 130018, China

Received:2005-12-30 Revised:1900-01-01 Online:2006-07-26 Published:2006-07-26
Contact: CHEN Xia

摘要/Abstract

摘要： 采用醇碱提取法提取黄芪多糖，通过单因素实验设计分别研究了提取溶剂pH值、料液比、提取时间、提取温度和提取溶剂乙醇浓度5种因素对黄芪多糖提取率的影响，利用紫外（可见）分光光度法和红外光谱法对醇碱提取法提取黄芪多糖的含量进行检测，计算黄芪多糖的提取率为19.15％， 分别是水提取法和碱提取法的3.53倍和2.63倍，残渣中有效成分的残留量低于其他两种提取方法.

关键词: 黄芪多糖, 醇碱提取法, 红外光谱法

Abstract: This paper deals with the ethanolalkali extraction method of astragalus polysaccharides, by which the effects of five extraction factors including pH of extraction solvent, the ratio of material to extraction solvent,extraction time, extraction temperature and ethanol concentration of extraction solvent on the rate of extraction were studied. The extraction rate of astragalus poly saccharides extracted by ethanolalkali extraction method was compared with those of water extraction method and alkaliextraction method and the content of astragaluspolysaccharides extracted by ethanolalkali extraction method was detected by ultraviolet spectrophotometry and infrared spectrometry. The results show that the extraction rate of ethanolalkali extraction method is 19.15%, 3.53 times that of water extraction method and 2.63 times that of alkali extraction method, the residue of the efficiency components of ethanolalkali extraction method is less than those of the other methods.

Key words: astragalus polysaccharides, ethanolalkali extraction method, infrared spectrometry

中图分类号:

Q946.3

田洛，宣依昉，范荣军，姜云垒，邢茗，刘立东，陈霞. 醇碱提取法提取黄芪多糖[J]. J4, 2006, 44(04): 652-657.

TIAN Luo, XUAN Yifang, FAN Rongjun, JIANG Yunlei, XING Ming, LIU Lidong, CHEN Xia. Ethanolalkali Extraction Method of Astragalus Polysaccharides[J]. J4, 2006, 44(04): 652-657.