以聚二烯丙基二甲基氯化铵为探针测定人血清中总蛋白的含量

以聚二烯丙基二甲基氯化铵为探针测定人血清中总蛋白的含量

郭晔, 陈艳华, 田媛, 高德江, 邓新煜, 张寒琦, 于爱民，盛韧

吉林大学化学学院, 长春 130012

收稿日期:2007-01-31 修回日期:1900-01-01 出版日期:2007-07-26 发布日期:2007-07-26
通讯作者: 于爱民

Determination of Protein in Human Serum Albumin withPoly(diallyldimethylammonium chloride) as Probe

GUO Ye, CHEN Yanhua, TIAN Yuan, GAO Dejiang, DENG Xinyu, ZHANG Hanqi, YU Aimin， SHENG Ren

College of Chemistry, Jilin University, Changchun 130012, China

Received:2007-01-31 Revised:1900-01-01 Online:2007-07-26 Published:2007-07-26
Contact: YU Aimin

摘要/Abstract

摘要： 通过共振光散射(RLS)技术, 将聚二烯丙基二甲基氯化铵(PDDA)作为探针应用于人血清中总蛋白含量的测定. 在一定的人血清白蛋白(HSA)浓度范围(2.35×10^-8-~1.17×10^-6mol/L)内, HSAPDDA体系的RLS强度随HSA浓度的增加而增加, 并具有一定的线性关系. 将该方法应用于实际人血清中总蛋白含量的测定, 其结果与考马士亮蓝法得到的结果进行对比, 较为满意.

关键词: 共振光散射, 聚二烯丙基二甲基氯化铵, 人血清白蛋白, 人血清, 总蛋白

Abstract: Based on the interaction of total protein with poly(diallyldimethylammonium chloride) (PDDA), a new method to determine the total protein in human serum was developed with the resonance light scattering (RLS) technique. Under appropriate conditions, the RLS intensity at the wavelength of 320 nm is directly proportional to the protein concentration in a range of 2.35×1 0^-8~1.17×10^-6 mol/L. The total protein in human serum was determined with the method mentioned above. The method was also compared with Bradford method and the results obtained were satisfactory.

Key words: resonance light scattering, poly(diallyldimethylam monium chloride), human serum albumin, human serum, total protein

中图分类号:

O657.3

郭晔, 陈艳华, 田媛, 高德江, 邓新煜, 张寒琦, 于爱民，盛韧. 以聚二烯丙基二甲基氯化铵为探针测定人血清中总蛋白的含量[J]. J4, 2007, 45(04): 673-675.

GUO Ye, CHEN Yanhua, TIAN Yuan, GAO Dejiang, DENG Xinyu, ZHANG Hanqi, YU Aimin， SHENG Ren. Determination of Protein in Human Serum Albumin withPoly(diallyldimethylammonium chloride) as Probe[J]. J4, 2007, 45(04): 673-675.

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