AFLP分子标记在鹀属遗传多样性分析中的初探

AFLP分子标记在鹀属遗传多样性分析中的初探

姜云垒¹，李时¹，王海涛²，高玮²

1. 吉林农业大学动物科学与技术学院，长春 130118； 2. 东北师范大学生命科学学院，长春 130024

收稿日期:2006-12-20 修回日期:1900-01-01 出版日期:2007-07-26 发布日期:2007-07-26
通讯作者: 姜云垒

AFLP Markers Analysis of Genetic Diversity in Emberiza

JIANG Yunlei¹， LI Shi¹， WANG Haitao²， GAO Wei²

1. College of Animal’s Science and Technology, Jilin Agricultural University, Changchun 130118, China; \=2. School of Life Sciences, Northeast Normal University, Changchun 130024, China

Received:2006-12-20 Revised:1900-01-01 Online:2007-07-26 Published:2007-07-26
Contact: JIANG Yunlei

摘要/Abstract

摘要： 利用扩增片断长度多态性（AFLP）技术分析了鹀属(Emberiza)4种鹀(三道眉草鹀Emberi za cioides, 白头鹀Emberiza leucocephala, 白眉鹀Emberiza tristrami, 灰头鹀Emberiza spodocephala)基因组DNA的多态性. 在选取的39对引物中, 有11对能得到重复性好的多态性扩增条带，每对引物扩增带数为27~76. 共得到535个标记位点, 其中429个为多态位点, 多态位点比率达801%; 在UPGMA聚类关系图中， 4种鹀聚成一大类，三道眉草鹀与白头鹀、白眉鹀与灰头鹀分别两两相聚. 结果表明， AFLP可用于分析鹀属遗传多样性.

关键词: 鹀, AFLP, 遗传多样性, 鸟类

Abstract: The amplified fragment length polymorphism (AFLP) technique was used to assess the genomic DNA polymorphism of four species Emberiza (cioides, leucocephala, tristrami and spodocephala)Eleven pairs out of thirtynine AFLP primer pairs produced reproducible polymorphic DNA amplification patterns.The amplified bands ranged from 27~76 per primer pair. Among the 535 AFLP markers scored, 429 ones werepolymorphic. The proportion of polymorphic loci was 801%. In UPGMA cluster analysis, four buntings were grouped into one branch, in which Emberiza cioidesand Emberiza leucocephala clustered together, and Emberiza tristramis and Emberiza spodocephala were grouped closely The result indicates that it is practical to reveal genetic diversity of Emberiza with AFLP markers.

Key words: bunting, AFLP, genetic diversity, birds

中图分类号:

Q959.7

姜云垒，李时，王海涛，高玮. AFLP分子标记在鹀属遗传多样性分析中的初探[J]. J4, 2007, 45(04): 686-690.

JIANG Yunlei， LI Shi， WANG Haitao， GAO Wei. AFLP Markers Analysis of Genetic Diversity in Emberiza[J]. J4, 2007, 45(04): 686-690.

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