盐胁迫条件下酿酒酵母的代谢通量分析

J4 ›› 2010, Vol. 07 ›› Issue (4): 699-703.

盐胁迫条件下酿酒酵母的代谢通量分析

李明达, 陈霞, 韩丹, 莫新迎, 赵长新

大连工业大学生物与食品工程学院, 辽宁省发酵工程重点实验室, 辽宁大连 116034

收稿日期:2009-12-08 出版日期:2010-07-26 发布日期:2011-06-14
通讯作者: 赵长新 E-mail:zhaochangxin@126.com

Metabolic Flux Analysis of Saccharomyces cerevisiae underSalt Stress Conditions

LI Mingda, CHEN Xia, HAN Dan, MO Xinying, ZHAO Changxin

Key Lab of Fermentation Engineering of Liaoning Province, School of Bio &|Food Technology ofDalian Polytechnic University, Dalian 116034, Liaoning Province, China

Received:2009-12-08 Online:2010-07-26 Published:2011-06-14
Contact: ZHAO Changxin E-mail:zhaochangxin@126.com

摘要/Abstract

摘要：

用计算代谢通量的方法研究酿酒酵母(Saccharomyces cerevisiae)在高盐胁迫条件下的生理代谢. 结果表明, 在盐胁迫条件下, 胞外海藻糖、甘油、乙醇和乳酸的质量浓度分别较常规培养条件下提高了24.44%,60.89%,23.32%和26.76%, 而柠檬酸、琥珀酸和苹果酸的质量浓度分别较常规培养条件下降低了82.11%,50.37%和53.33%. 代谢通量分析表明, 在盐胁迫培养条件下糖酵解途径各支路的代谢产物通量均有所增加, 而TCA循环中各代谢产物的通量均有所减少.

关键词: 酿酒酵母；盐胁迫；代谢流分析

Abstract:

A method to calculate the metabolic flux of Saccharomyces cerevisiae metabolism was studied under salt stress conditions. The result shows that under salt stress, the contents of trehalose, glycerol, ethanol and lactate were increased by 24.44%, 60.89%, 23.32% and 26.76%, respectively, the contents of citrate, succinate and fumarate were decreased by 82.11%, 50.37% and 53.33%, respectively. The metabolic flux analysis indicates that the metabolic product flux of each branch channel in EMP was raised at the culture condition of the salt stress, in contrast, each metabolic flux of TCA cycle was reduced.

Key words: Saccharomyces cerevisiae, salt stress, metabolic flux analysis

中图分类号:

Q332

李明达, 陈霞, 韩丹, 莫新迎, 赵长新. 盐胁迫条件下酿酒酵母的代谢通量分析[J]. J4, 2010, 07(4): 699-703.

LI Meng-Da, CHEN Xia, HAN Dan, MO Xin-Ying, DIAO Chang-Xin. Metabolic Flux Analysis of Saccharomyces cerevisiae underSalt Stress Conditions[J]. J4, 2010, 07(4): 699-703.