长白山林蛙输卵管糖蛋白的分离鉴定

J4 ›› 2011, Vol. 49 ›› Issue (02): 336-339.

长白山林蛙输卵管糖蛋白的分离鉴定

郑明珠, 方丽, 吕萍, 陈亮, 刘景圣

吉林农业大学食品科学与工程学院, 长春 130118

收稿日期:2010-05-27 出版日期:2011-03-26 发布日期:2011-06-14
通讯作者: 刘景圣 E-mail:liujs1007@vip.sina.com.cn

Separation and Identification of Rana c ChangbaishanensisOviduct Glycoprotein

ZHENG Mingzhu, FANG Li, LV Ping, CHEN Liang, LIU Jingsheng

College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China

Received:2010-05-27 Online:2011-03-26 Published:2011-06-14
Contact: LIU Jingsheng E-mail:liujs1007@vip.sina.com.cn

摘要/Abstract

摘要：

以长白山林蛙为研究对象, 对其输卵管糖蛋白进行提取分离及鉴定. 长白山林蛙输卵管冲洗液经饱和硫酸铵沉淀, DEAE-Cellulose 52离子交换柱, Sephadex G-100凝胶柱层析纯化, 得到蛋白质和糖的重合峰, 经透析、冷冻干燥得长白山林蛙输卵管糖蛋白纯品OGP-Ⅰ. SDSPAGE电泳实验结果表明, OGP-Ⅰ是分子量为116 000的均一带. 对其分别进行蛋白和糖染色, 同一位置都出现单一条带；紫外全扫描OGP-Ⅰ有多糖特征峰和蛋白吸收峰, 表明OGP-Ⅰ是糖蛋白纯品.

关键词: 长白山林蛙；输卵管糖蛋白；分离；纯化；鉴定

Abstract:

Rana chensinensis Changbaishanensis oviduct glycoprotein was separated and identified in the test. Saturated ammonium sulfate was uesd to pre
cipitate Rana c Changbaishanensis oviduct glycoprotein(the optimization saturated degree of saturated ammonium sulfate is 70%), followed by DEAE Cellulose52 ion exchange chromatography initial purification, then Sephadex G100 further purification.To collect the overlap peak of protein and sugar. It’s the Rana c. Changbaishanensis oviduct glycoprotein sterling(OGP-Ⅰ) after lyophillization. The result of SDSPAG electrophoresis indicates OGP-Ⅰ is only a colored zone. The molecular weight of glycoprotein was 116 000. The result of SDSPAG electrophoresis combined with coomassie brilliant blue staining and shiff staining provs it is glycoprotein, OGP-Ⅰ is only a colored zone at the same local. The ultraviolet scanning of OGP-Ⅰ indicates that it exhibits polysaccharide characteristic absorption band and protein absorption band. Therefore
, these can further indicate that OGP-Ⅰ is glycoprotein.

Key words: Rana chensinensis Changbaishanensis, oviduct glycoprotein, separation, purification, identify

中图分类号:

Q513.2

郑明珠, 方丽, 吕萍, 陈亮, 刘景圣. 长白山林蛙输卵管糖蛋白的分离鉴定[J]. J4, 2011, 49(02): 336-339.

ZHENG Meng-Zhu, FANG Li, LV Ping, CHEN Liang, LIU Jing-Ku. Separation and Identification of Rana c ChangbaishanensisOviduct Glycoprotein[J]. J4, 2011, 49(02): 336-339.