顶空气相色谱法测定人参根皂苷中的大孔吸附树脂残留物

J4 ›› 2011, Vol. 49 ›› Issue (02): 343-345.

顶空气相色谱法测定人参根皂苷中的大孔吸附树脂残留物

宋利捷¹, 郑波², 陈新³

1. 吉林省中医药科学院药剂科, 长春 130021； 2. 白求恩医科大学制药厂, 长春 130012;3. 吉林省消防总队医院, 长春 130061

收稿日期:2010-08-30 出版日期:2011-03-26 发布日期:2011-06-14
通讯作者: 宋利捷 E-mail:476139482@qq.com

Determination of Macroporous Resin Residua in Saponins of the Roots of Panax ginseng C A Meyer by HeadspaceGC

SONG Lijie¹, ZHENG Bo², CHEN Xin³

1. Department of Pharmacy, Jilin Academy of Chinese Medical Sciences, Changchun 130021, China；2. Pharmaceutical Factory of Bethune University of Medical Sciences, Changchun 130012, China;3. Fire Corps Hospital of Jilin Province, Changchun 130061, China

Received:2010-08-30 Online:2011-03-26 Published:2011-06-14
Contact: SONG Lijie E-mail:476139482@qq.com

摘要/Abstract

摘要：

采用顶空气相色谱法测定人参根皂苷中大孔树脂残留物的质量浓度. 色谱柱初始温度60 ℃, 保持22 min, 以50 ℃/min升至200 ℃, 保持6 min, 氢火焰离子检测器温度为260 ℃, 进样口温度220 ℃；用质量分数为40%的N,N二甲基甲酰胺作溶剂, 载气为氮气. 实验结果表明, 正己烷、苯、甲苯、对二甲苯、苯乙烯、对二乙基苯6种大孔树脂残留物在各自的质量浓度范围内呈良好的线性关系, 且回收率符合要求.

关键词: 人参根皂苷；大孔吸附树脂；残留物；顶空气相色谱法

Abstract:

To establish headspaceGC method for the determination of residua of macroporous resin in the saponins extracted from the roots of Panax ginseng C A Meyer, the chromatographic separation was performed. The column temperature was programed, with an initial temperature of 60 ℃ (hold 22 min), at an increasing rate of 50 ℃/min to 200 ℃ (hold 6 min); the injector temperature was set at 220 ℃ and FID detector temperature was set at 260 ℃; solvent was 40% N,NDimethyl acetamide (DMF)； the carrier gas was nitrogen. All the residua including nhexane, benzene, toluene, 1,4xylene, styrene and 1,4diethylbenzene gave good linearities and the recoveries.

Key words: saponins of the roots of Panax ginseng C A Meyer, macroporous resin； residua； headspaceGC

中图分类号:

R917

宋利捷, 郑波, 陈新. 顶空气相色谱法测定人参根皂苷中的大孔吸附树脂残留物[J]. J4, 2011, 49(02): 343-345.

SONG Li-Cha, ZHENG Bei, CHEN Xin. Determination of Macroporous Resin Residua in Saponins of the Roots of Panax ginseng C A Meyer by HeadspaceGC[J]. J4, 2011, 49(02): 343-345.