平板扩散法测定黄嘌呤氧化酶的活性

J4 ›› 2011, Vol. 49 ›› Issue (03): 544-547.

平板扩散法测定黄嘌呤氧化酶的活性

李忠琴¹, 杨元营², 许小平²

1. 集美大学水产学院, 福建厦门 361021|2. 福州大学化学化工学院, 福州 350002

收稿日期:2010-08-04 出版日期:2011-05-26 发布日期:2011-06-15
通讯作者: 许小平 E-mail:xu@fzu.edu.cn

Plate Diffusion Assay for Xanthine Oxidase Activity

LI Zhongqin¹, YANG Yuanying², XU Xiaoping²

1. College of Fisheries, Jimei University, Xiamen 361021, Fujian Province, China；2. College of Chemistry and Chemical Engineeing, Fuzhou University, Fuzhou 350002, China

Received:2010-08-04 Online:2011-05-26 Published:2011-06-15
Contact: XU Xiaoping E-mail:xu@fzu.edu.cn

摘要/Abstract

摘要：

通过对Arthrobacter- sp.X11菌株所产黄嘌呤氧化酶(XOD)活性测定方法的研究和比较, 建立一种简便可行的黄嘌呤氧化酶活性快速检测方法, 即平板扩散法. 实验结果表明, 该方法测得的显色圈直径与辣根过氧化物酶分光光度法测得的酶活力之间有良好的线性关系, 通过测量显色圈的直径即可估算出样品的酶活力.

关键词: 黄嘌呤氧化酶；平板扩散法；显色圈直径；氮源

Abstract:

Compared with spectrophotometry for the determination of xanthine oxidase activity by horseradish peroxidase, a quick and simple solid agar plate method of assaying the activity of xanthine oxidase(XOD) produced by Arthrobacter sp.X11 was developed with xanthine as a substrate. The activity of xanthine oxidase was analyzed quickly and roughly according to the diameters of color haloes. This method was used to optimize nitrogen source in a fermentation medium for enzyme production.

Key words: xanthine oxidase, plate diffusion method, diameter of color halo, nitrogen source

中图分类号:

李忠琴, 杨元营, 许小平. 平板扩散法测定黄嘌呤氧化酶的活性[J]. J4, 2011, 49(03): 544-547.

LI Zhong-Qin, YANG Yuan-Ying, HU Xiao-Beng. Plate Diffusion Assay for Xanthine Oxidase Activity[J]. J4, 2011, 49(03): 544-547.