一株极端耐盐曲霉的分离、 鉴定及生物学特性分析

J4 ›› 2011, Vol. 49 ›› Issue (03): 548-553.

一株极端耐盐曲霉的分离、鉴定及生物学特性分析

刘晓丹^1,2, 刘金亮¹, 魏毅¹, 田玉平¹, 范飞飞¹, 潘洪玉¹, 张世宏¹

1. 吉林大学植物科学学院, 长春 130062； 2. 吉林农业科技学院生物工程学院, 吉林吉林 132101

收稿日期:2010-06-01 出版日期:2011-05-26 发布日期:2011-06-15
通讯作者: 张世宏 E-mail:zhang_sh@jlu.edu.cn

solation, Identification and Biologic Characteristics of an Extreme Halotolerant Aspergillus sp.

LIU Xiaodan^1,2, LIU Jinliang¹, WEI Yi¹, TIAN Yuping¹, FAN Feifei¹,PAN Hongyu¹, ZHANG Shihong¹

1. College of Plant Sciences, Jilin University, Changchun 130062, China;2. School of Biological Engineering, Jilin Agricultural Science and Technology College, Jilin 132101, Jilin Province, China

Received:2010-06-01 Online:2011-05-26 Published:2011-06-15
Contact: ZHANG Shihong E-mail:zhang_sh@jlu.edu.cn

摘要/Abstract

摘要：

从晒盐场盐池周边的风干野生植被表面分离筛选到1株极端耐盐菌株CCHA, 通过形态观察及ITS序列分析对菌株进行鉴定, 并对其生物学特性、耐盐性及金属耐受性进行分析. 研究结果表明: 菌株CCHA与 21株己报道的曲霉属菌株同源性为55.9%~100%；其生长温度范围为25~45 ℃, 最适温度为35 ℃；生长pH值范围为2.5~12.0, 最适pH值为5.0~8.0；生长盐度范围为0~31%, 最适盐度为5%；对Cu²⁺有较强的耐受性. 鉴定该菌株为曲霉菌（Aspergillus sp.）.

关键词: 耐盐曲霉；分离；鉴定；转录间隔区(ITS)；盐度； pH值；金属耐受性

Abstract:

A halotolerant strain CCHA was isolated from the surface of wild vegetation around saltern, identified by the morphological observation and ITS sequence analysis, and their biological characteristics, saltresistance and metaltolerance were analyzed. The homology of ITS sequence between CCHA and each of other 21 Aspergillus strains which have been reported was 55.9%—100%; growth temperature range of it was from 25 ℃ to 45 ℃, the optimum at 35 ℃; growth pH range of it was from 2.5 to 12.0, the optimum at 5.0—8.0; salinity range of it was 0—31%, the optimum at 5%; and the strain exhibited higher tolerance to Cu2+. CCHA strain was identified as Aspergillus sp.

Key words: halotolerant Aspergillus sp., isolation, identification, internal transcribed spacer(ITS), salinity, pH value, metalresistance

中图分类号:

Q939

刘晓丹, 刘金亮, 魏毅, 田玉平, 范飞飞, 潘洪玉, 张世宏. 一株极端耐盐曲霉的分离、鉴定及生物学特性分析[J]. J4, 2011, 49(03): 548-553.

LIU Xiao-Dan, LIU Jin-Liang, WEI Yi, TIAN Yu-Beng, FAN Fei-Fei, BO Hong-Yu, ZHANG Shi-Hong. solation, Identification and Biologic Characteristics of an Extreme Halotolerant Aspergillus sp.[J]. J4, 2011, 49(03): 548-553.