人参SQS基因的干扰载体构建及转化人参愈伤组织

J4 ›› 2011, Vol. 49 ›› Issue (06): 1136-1140.

人参SQS基因的干扰载体构建及转化人参愈伤组织

蒋世翠¹, 张明哲¹, 王义¹, 孙春玉¹, 李校堃^1,2, 张美萍^1

1. 吉林农业大学生命科学学院, 长春 130118;2. 吉林农业大学生物反应器与药物开发教育部工程中心, 长春 130118

收稿日期:2010-12-29 出版日期:2011-11-26 发布日期:2011-11-28
通讯作者: 张美萍 E-mail:wzhaoyun@tom.com

Interference Vector Construction of Panax ginseng’s SQS Gene and Transformation Callus

JIANG Shicui¹, ZHANG Mingzhe¹, WANG Yi¹, SUN Chunyu¹, LI Xiaokun^1,2, ZHANG Meiping¹

1. College of Life Science, Jilin Agricultural University, Changchun 130118, China|2. Engineering Research Center ofBioreactor and Pharmaceutical Development of Ministry of Education, Jilin Agricultural University, Changchun 130118, China

Received:2010-12-29 Online:2011-11-26 Published:2011-11-28
Contact: ZHANG Meiping E-mail:wzhaoyun@tom.com

摘要/Abstract

摘要：

以5年生人参为材料, 根据人参SQS基因设计正义及反义片段引物, 构建了SQS基因干扰表达载体；利用农杆菌转化法转化人参愈伤组织, 对获得的抗性愈伤组织进行PCR检测及实时定量PCR检测, 并对抗性愈伤组织的皂苷进行HPLC分析. 实验结果表明, 与非转化人参愈伤组织相比, 转化后的愈伤组织SQS基因表达量降低, 皂苷含量有所变化. SQS是人参皂苷生物合成途径的关键酶, 抑制SQS基因的表达, 可调控人参皂苷含量变化.

关键词: 人参, 干扰载体, SQS基因, 人参愈伤组织

Abstract:

According to the SQS gene of Panax ginseng, the authors designed a sense primer and an antisense primer to construct a SQS interference expression vector. The ginseng callus was transformed by agrobacteriummediated methods, and the selected resistant callus was assayed by PCR and realtime PCR, and the ginsenosides of resistant callus was analyzed by HPLC. Compared with the nontransformation ginseng callus, the SQS gene’s expression of positive callus decreased, and the ginsenosides also had certain changes. SQS is a critical biosynthetic enzyme of ginsenoside. To inhibit the expression of SQS can mediate the change of the content of the ginsenosides of ginseng.

Key words: Panax ginseng; , interference vector, SQS gene, callus of Panax ginseng

中图分类号:

Q786

蒋世翠, 张明哲, 王义, 孙春玉, 李校堃, 张美萍. 人参SQS基因的干扰载体构建及转化人参愈伤组织[J]. J4, 2011, 49(06): 1136-1140.

JIANG Shi-Cui, ZHANG Meng-Zhe, WANG Xi, SUN Chun-Yu, LI Jiao-Kun, ZHANG Mei-Ping. Interference Vector Construction of Panax ginseng’s SQS Gene and Transformation Callus[J]. J4, 2011, 49(06): 1136-1140.

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