平菇多糖提取方法的比较

J4 ›› 2011, Vol. 49 ›› Issue (06): 1141-1149.

平菇多糖提取方法的比较

张敏¹, 陈瑞战¹, 陈瑞平¹, 刘志强², 孟繁磊³, 李元¹, 董航¹

1. 长春师范学院化学学院, 长春 130032； 2. 中国科学院长春应用化学研究所质谱中心, 长春 130022；3. 吉林省农业科学院, 长春 130033

收稿日期:2011-02-20 出版日期:2011-11-26 发布日期:2011-11-28
通讯作者: 陈瑞战 E-mail:rzchen@yeah.net

Comparison among Extraction Technologies of Polysaccharidefrom Pleurotus ostreatus

ZHANG Min¹, CHEN Ruizhan¹, CHEN Ruiping¹, LIU Zhiqiang^2,MENG Fanlei³, LI Yuan¹, DONG Hang¹

1. College of Chemistry, Changchun Normal University, Changchun 130032, China|2. Center of Mass Spectrometry,Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;3. Jilin Academy of Agricultural Sciences, Changchun 130033, China

Received:2011-02-20 Online:2011-11-26 Published:2011-11-28
Contact: CHEN Ruizhan E-mail:rzchen@yeah.net

摘要/Abstract

摘要：

以平菇为原料, 分别采用破碎、超声波和超声辅助复合酶3种提取方法提取平菇水溶性多糖. 正交优化结果表明：提取温度90 ℃, 剪切速率11 350 r/min, 提取时间20 min, 提取3次, 破碎法提取多糖得率为11.20%；提取温度60 ℃, 超声功率450 W, 提取时间60 min, 提取3次, 超声法提取多糖得率为9.75%；提取温度30 ℃, 超声功率350 W, 提取时间60 min, 溶液pH=5, 超声辅助复合酶法提取多糖得率为12.05%. 综合考虑提取得率、节能环保、操作方法等因素, 选用破碎法提取平菇多糖较理想.

关键词: 平菇多糖；破碎提取；超声波提取；超声辅助复合酶；正交设计试验

Abstract:

The Pleurotus ostreatus were used as the raw material for extracting polysaccharide by means of smashing extractor, ultrasonic extraction, ultrasonicassisted complex enzyme extraction. The results indicate the optimal extraction conditions of the smashing extractor were extraction temperature 90 ℃, extraction rate 11 350 r/min, extraction time 20 min, extraction 3 times, and the yield of polysaccharide extracted was 11.20%. The optimal extraction conditions of ultrasonic extraction were extraction temperature 60 ℃, extraction time 60 min, ultrasonic power 450 W, extraction 3 times, and the yield of polysaccharide extracted was 9.75%. The optimal extraction conditions of ultrasonicassisted complex enzyme extraction were extraction temperature 30 ℃, extraction time 60 min, ultrasonic power 350 W, extraction pH 5, the yield of polysaccharide extracted was 1205%. In comprehensive consideration of the yield of polysaccharide extracted, saving energy, simple operation, the smashing extractor was considered as an ideal method.

Key words: Pleurotus ostreatus polysaccharide, smashing extraction; , ultrasonic extraction, ultrasonicassisted complex enzyme;orthogonal array design

中图分类号:

R284.2

张敏, 陈瑞战, 陈瑞平, 刘志强, 孟繁磊, 李元, 董航. 平菇多糖提取方法的比较[J]. J4, 2011, 49(06): 1141-1149.

ZHANG Min, CHEN Rui-Zhan, CHEN Rui-Beng, LIU Zhi-Jiang, MENG Fan-Lei, LI Yuan, DONG Hang. Comparison among Extraction Technologies of Polysaccharidefrom Pleurotus ostreatus[J]. J4, 2011, 49(06): 1141-1149.

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