J4 ›› 2013, Vol. 51 ›› Issue (01): 148-152.

• 环境科学 • 上一篇    下一篇

石油污染地下水中苯降解菌的代谢特征

金欢驰, 张玉玲, 党江艳   

  1. 吉林大学 环境与资源学院, 水资源与环境研究所, 长春 130012
  • 收稿日期:2012-04-24 出版日期:2013-01-26 发布日期:2013-01-31
  • 通讯作者: 张玉玲 E-mail:zhangyl8@jlu.edu.cn

Metabolic Characteristics of Benzene by Strain fromPetroleumContaminated Groundwater

JIN Huanchi, ZHANG Yuling, DANG Jiangyan   

  1. College of Environment and Resources, Institute of Water Resources and Environment,Jilin University, Changchun 130012, China
  • Received:2012-04-24 Online:2013-01-26 Published:2013-01-31
  • Contact: ZHANG Yuling E-mail:zhangyl8@jlu.edu.cn

摘要:

从东北某油区石油污染的地下水中分离、 纯化出苯降解优势菌B6, 研究其降解效率和代谢特征. 结果表明: 当模拟石油污染地下水中苯的质量浓度
为394.36 mg/L时, 3 d后的降解率为82.82%; 相关酶活及代谢产物在双加氧酶的作用下, B6菌降解苯的途径为儿茶酚正位裂解; B6菌在代谢过程中产生色氨酸酶、 脂肪酶、 淀粉酶、 明胶酶、 乳糖酶和氧化酶等, 葡萄糖、 乳糖、 蛋白质、 色氨酸、 脂肪、 淀粉、 明胶、 多肽、 纤维素、 含硫有机物和细胞色素C等为其营养物质, 以亚硝酸盐和硝酸盐为电子受体; B6菌为革兰氏阳性红平红球菌.

关键词: 石油污染地下水; 苯; 降解菌; 代谢

Abstract:

The authors investigated the degradation features of benzene by strain B6 dominant strain isolated and purified from the petroleumcontaminated groundwater in the northeast of China. Results show that when the initial mass concentration of benzene was 394.36 mg/L, the degradation rate of benzene by train B6 was 82.82% on the third day; ortho 2 cleavage of catechol was the right approach of benzene degradation under the effect of dioxygenase; B6 strain could produce tryptophanase, lipase, amylases, gelatinase, lactase, oxidase, use glucose, lactose, protein, tryptophan, fat, starch, gelatine, polypeptide, cellulose, sulfurcontaining organic compounds, cytochrome C as nutrients, and utilize nitrites and nitrates as electron acceptors in its metabolic process. It was identified that B6 strain was Rhodococcus erythropolis, grampositive bacterium.

Key words: petroleumcontaminated groundwater, benzene, degradation strain, metabolism

中图分类号: 

  • X523