羊传染性脓疱病毒吉林分离株F1L基因的克隆与同源性分析

J4 ›› 2013, Vol. 51 ›› Issue (03): 518-522.

羊传染性脓疱病毒吉林分离株F1L基因的克隆与同源性分析

邵洪泽^1,2, 程荣华², 石春军³, 呼延含蓉², 黄海楠², 许尧², 毛文智², 胡桂学¹

1. 吉林农业大学动物科技学院, 长春 130118； 2. 吉林省畜牧兽医科学研究院, 长春 130062；3. 前郭县动物疫病预防控制中心, 吉林松原 131100

收稿日期:2012-08-11 出版日期:2013-05-26 发布日期:2013-05-17
通讯作者: 胡桂学 E-mail:huguixue901103@163.com

Cloning and Homology Analysis of F1L Gene ofOrf virus Isolated from Jilin Strain

SHAO Hongze^1,2, CHENG Ronghua², SHI Chunjun³, HUYAN Hanrong²,HUANG Hainan², XU Yao², MAO Wenzhi², HU Guixue¹

1. College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China;2. Academy of Animal Science and Veterinary Medicine of Jilin Province, Changchun 130062, China;3. Animal Disease Control Center of Qianguo Country, Songyuan 131100, Jilin Province, China

Received:2012-08-11 Online:2013-05-26 Published:2013-05-17
Contact: HU Guixue E-mail:huguixue901103@163.com

摘要/Abstract

摘要：

通过参考GenBank中羊传染性脓疱病毒的F1L基因序列设计引物, 应用PCR技术的特异性扩增了羊传染性脓疱病毒JLSY04株的F1L基因片段, 测序得到了该病毒F1L基因序列, 并与几个参考毒株序列进行比对. 实验结果表明, 羊传染性脓疱病毒JLSY04株与OV/Torino株同源性最低, 为96.0％, 与Jilin株同源性最高, 为99.4%. 即该羊传染性脓疱病毒JLSY04株F1L基因与其他参考毒株间的差异较小, 可作为基因工程疫苗的目的基因.

关键词: 羊传染性脓疱病毒； F1L基因；克隆；同源性分析

Abstract:

According to the DNA sequence of the F1L gene which was stored in GenBank, the authors designed one pair of primers to amplify F1L gene of JLSY04 by PCR. The amplified positive production was sequenced and the sequence of it was compared with the sequences of several reference strains domesticisolated. The results show that JLSY04 stain and OV/Torino strain show the lowest homology of 960% with respect to F1L gene, and the highest homology of Jilin strain was up to 994%. There was little difference of F1L gene between JLSY04 strain and each of other reference strains isolated from different species and different areas in China. The analysis result of homology suggests that the F1L gene can be used as the target genes of the genetically engineered vaccine.

Key words: Orf virus, F1L gene, cloning, homology analysis

中图分类号:

邵洪泽, 程荣华, 石春军, 呼延含蓉, 黄海楠, 许尧, 毛文智, 胡桂学. 羊传染性脓疱病毒吉林分离株F1L基因的克隆与同源性分析[J]. J4, 2013, 51(03): 518-522.

SHAO Hong-Ze, CHENG Rong-Hua, DAN Chun-Jun, HE Yan-Han-Rong, HUANG Hai-Nan, HU Yao, MAO Wen-Zhi, HU Gui-Hua. Cloning and Homology Analysis of F1L Gene ofOrf virus Isolated from Jilin Strain[J]. J4, 2013, 51(03): 518-522.