Meso-四(对-羟基苯基)卟啉和溶菌酶的相互作用机理

吉林大学学报(理学版)

Meso-四(对-羟基苯基)卟啉和溶菌酶的相互作用机理

赵婷婷¹, 毕淑云¹, 王瑜¹, 王天娇¹, 庞博²

1. 长春师范大学化学学院, 长春 130032； 2. 吉林出入境检验检疫局检疫检验技术中心, 长春 130062

收稿日期:2013-12-09 出版日期:2014-07-26 发布日期:2014-09-26
通讯作者: 毕淑云 E-mail:sy_bi@sina.com

Interaction Mechanism of Mesotetra(4hydroxyphenyl)porphyrinand Lysozyme

ZHAO Tingting^1, BI Shuyun^1, WANG Yu^1, WANG Tianjiao^1, PANG Bo^2

1. College of Chemistry, Changchun Normal University, Changchun 130032, China; 2. Technology Centerof Inspection and Quarantine, Jilin EntryExit Ins
pection and Quarantine Bureau, Changchun 130062, China

Received:2013-12-09 Online:2014-07-26 Published:2014-09-26
Contact: BI Shuyun E-mail:sy_bi@sina.com

摘要/Abstract

摘要：

在模拟生理条件下, 利用荧光光谱和同步荧光光谱法研究Meso-四(对-羟基苯基)卟啉(THPP)与溶菌酶的相互作用. 结果表明: THPP对溶菌酶的猝灭过程为静态猝灭; 291 K时, THPP与溶菌酶的结合常数为5.97×10⁴ L/mol; 疏水作用力为THPP与溶菌酶的主要作用力; 金属离子Fe²⁺,Fe³⁺,Cu²⁺,Mg²⁺,Ca²⁺和Zn²⁺的存在不影响THPP与溶菌酶的结合常数; THPP与溶菌酶的结合距离为3.35 nm; THPP可使色氨酸残基周围的极性减弱, 疏水性增强.

关键词: meso四(对-羟基苯基)卟啉, 溶菌酶, 荧光猝灭

Abstract:

Meso-tetra(4-hydroxyphenyl)porphyrin (THPP) interacting with lysozyme was investigated by the fluorescence spectroscopy under simulate
d physiological conditions. The results indicate that THPP quenched the intrinsic fluorescence of lysozyme via a static quenching procedure. The binding constant KA between THPP and lysozyme was 5.97×104 L/mol at 291 K. According to the thermodynamic parameters, it was obtained that hydrophobic was a main acting force between THPP and lysozyme. The presence of Fe²⁺,Fe³⁺,Cu²⁺,Mg²⁺,Ca²⁺and Zn²⁺ did not affect the binding constant of THPP and lysozyme. According to Frster’s nonradiative energy transfer theory, the distance between THPP and lysozyme was 3.35nm. The synchronous fluorescence spectra reveal that the vicinity of tryptophan becomes more hydrophobicity with the increase of THPP.

Key words: meso-tetra(4-hydroxyphenyl)porphyrin (THPP), lysozyme, fluorescence quenching

中图分类号:

O657.3

赵婷婷, 毕淑云, 王瑜, 王天娇, 庞博. Meso-四(对-羟基苯基)卟啉和溶菌酶的相互作用机理[J]. 吉林大学学报(理学版), 2014, 52(04): 820-824.

ZHAO Tingting, BI Shuyun, WANG Yu, WANG Tianjiao, PANG Bo. Interaction Mechanism of Mesotetra(4hydroxyphenyl)porphyrinand Lysozyme[J]. Journal of Jilin University Science Edition, 2014, 52(04): 820-824.

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