HPLC-ELSD法测定珠子参根茎中新化合物24（R）-珠子参苷R1的质量比

吉林大学学报(理学版)

HPLC-ELSD法测定珠子参根茎中新化合物24（R）-珠子参苷R₁的质量比

王加付^1,2, 彭绍¹, 李兰杰¹, 杨洁¹, 孙婷¹, 李绪文¹, 金永日¹, 时晓磊^1,3

1. 吉林大学化学学院, 长春 130012; 2. 黑龙江烟草有限责任公司, 哈尔滨 150001;3. 吉林大学军需科技学院, 长春 130062

收稿日期:2013-10-24 出版日期:2014-09-26 发布日期:2014-09-26
通讯作者: 时晓磊 E-mail:xlshi@jlu.edu.cn

Determination of New Compound 24(R)-Majoroside R₁in Panax japonicus C.A.Mey.var.Major（Burk.[KG-*3]） C.Y.Wu et K.M.Feng by H
PLCELSD

WANG Jiafu^1,2, PENG Shao¹, LI Lanjie¹, YANG Jie¹, SUN Ting^1, LI Xuwen¹, JI Yongri¹, SHI Xiaolei^1,3

1. College of Chemistry, Jilin University, Changchun 130012, China;2. Heilongjiang Tobacco Limited Liability Company, Harbin 150001, China;3. College of Quartermaster Technology, Jilin University, Changchun 130062, China

Received:2013-10-24 Online:2014-09-26 Published:2014-09-26
Contact: SHI Xiaolei E-mail:xlshi@jlu.edu.cn

摘要/Abstract

摘要：

采用高效液相色谱蒸发光散射法(HPLC\|ELSD)测定珠子参根茎中新化合物24（R）-珠子参苷R₁的质量浓度. 色谱条件为： Aglilent Zorbax SBC₁₈（ODS, 4.6 mm×250 mm, 5 μm）色谱柱, 流动相V(甲醇)∶V(水)=58∶42, 流速1.0 mL/min, 柱温25 ℃, 载气压力1.94×105 Pa, 漂移管温度85 ℃, 撞击器关闭, 进样量20 μL. 结果表明：在0.40~20.0 μg内, 24（R）珠子参苷R1线性关系较好, r=0.999 3（n=6）; 24（R）珠子参苷R₁的平均回收率为98.7%, RSD=1.28%（n=6）.

关键词: 24（R）-珠子参苷R₁, 高效液相色谱蒸发光散射法, 质量比

Abstract:

The determination of the new compound of 24(R)majoroside R₁ in Panax japonicus C.A. Mey. var. Major (Burk.[KG-*3]) C.Y. Wu et
K. M. Feng was developed by HPLC\|ELSD method. The conditions of chromatography were Aglient Zorbax SBC₁₈ Column (ODS, 4.6 mm×250 mm, 5 μm) was adopted; mobile phase was composed of V(methanol)∶V(water)=58∶42; flow rate was set at 1.0 mL/min; temperature of column was set at 25 ℃; carrier gas pressure was set at 1.94×105 Pa tube temperature of ELSD was set at 85 ℃; impactor was set in closed state. As the results show in the range of 0.4—20.0 μg, the linearity of 24(R)majoroside R1 is good, r=0.999 3(n=6), the average recovery was 98.7%, and RSD=1.28% (n=6).

Key words: 24(R)majoroside R1, high performance liquid chromatography-evaporative light scatteringdatection (HPLCELSD), mass ratio

中图分类号:

R917

王加付, 彭绍, 李兰杰, 杨洁, 孙婷, 李绪文, 金永日, 时晓磊. HPLC-ELSD法测定珠子参根茎中新化合物24（R）-珠子参苷R₁的质量比[J]. 吉林大学学报(理学版), 2014, 52(05): 1095-1099.

WANG Jiafu, PENG Shao, LI Lanjie, YANG Jie, SUN Ting, LI Xuwen, JI Yongri, SHI Xiaolei. Determination of New Compound 24(R)-Majoroside R₁in Panax japonicus C.A.Mey.var.Major（Burk.[KG-*3]） C.Y.Wu et K.M.Feng by H
PLCELSD[J]. Journal of Jilin University Science Edition, 2014, 52(05): 1095-1099.

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HPLC-ELSD法测定珠子参根茎中新化合物24（R）-珠子参苷R₁的质量比

Determination of New Compound 24(R)-Majoroside R₁in Panax japonicus C.A.Mey.var.Major（Burk.[KG-*3]） C.Y.Wu et K.M.Feng by H
PLCELSD

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