关键词: 硝苯地平, 脂质体, 乙醚注入法, BoxBehnken, 人工神经网络

Abstract:

The nifedipine liposome was prepared from an appropriate amount of lecithine, cholesterol and nifedipine by the ether injection method. On the basis of single factor experiment, we used statistical methods to investigate the effects of phospholipid amount, lipid drug ratio, cholesterol phospholipid ratio on the encapsulation efficiency of liposome. The results show that the encapsulation rate of the liposome prepared based on multivariate quadratic regression model was 8654%, and the encapsulation rate of the liposome prepared based on artificial neural network model was 9725%. Compared with the quadratic regression model, artificial neural network model combined with genetic algorithm is more suitable for optimizing the preparation technique of nifedipine liposome. After optimizing, the best preparation formulation is 612.4 mg of phospholipid with a lipid drug ratio of 60.02 and a cholesterol phospholipid ratio of 6.401.

Key words: nifedipine, liposome, ether injection method, Box-Behnken, artificial neural network