关键词: 蜜环菌, β-1,6-葡聚糖, 人源巨噬细胞极化, 结肠癌

Abstract: M2-like macrophages induced from human macrophages THP-1 were used as the model, we studied  inhibitory effects of β-1,6-glucan   isolated and purified from Armilla melariella (AAMP-A70) on its polarization and analyzed the role and mechanism of AAMP-A70 in inhibiting DLD-1 proliferation of colon cancer cells by regulating macrophage polarization.   The enzyme linked immunosorbent assay (ELISA) and quantitative real-time PCR (qRT-PCR)  were used to detect the expression of M1-like and M2-like macrophage markers in M2 macrophages induced by THP-1 before and after AAMP-A70 treatment, DLD-1 cells were treated with cell culture medium of each treatment group, and the proliferation of DLD1 cells was detected by MTT assay and crystal violet staining assay, and the expression of apoptosis protein in DLD-1 cells was detected by Western-blot test.  The experimental results show that AAMP-A70 can significantly inhibit the expression of M2-like macrophages markers including VEGF,Arg-1 and TGF-β, and promote the expression of M1-like macrophages markers including TNF-α,IL-1-β and CCR7. The  culture medium of AAMP-A70 treated cells can significantly inhibit the  proliferation of DLD-1 in a concentration-dependent manner.  The supernatant of AAMP-A70 treated cells can induce up regulation of cleaved-PARP and cleaved-Caspase-3 expressed in DLD-1 cells.

Key words: Amillariella mellea,  , β-1,6-glucan,  , human macrophages polarization,  , colon cancer