Se-scFv-B3对过氧化氢诱导的大鼠乳鼠心肌细胞损伤的保护作用

J4 ›› 2010, Vol. 48 ›› Issue (1): 146-151.

Se-scFv-B3对过氧化氢诱导的大鼠乳鼠心肌细胞损伤的保护作用

苏家明^1,2, 魏景艳¹, 宋宇³, 杨蕾⁴, 霍锐¹

1. 吉林大学药学院, 长春 130021； 2. 吉林大学生命科学学院, 长春 130021；3. 吉林大学畜牧兽医学院, 长春 130062； 4. 吉林大学中日联谊医院, 长春 130033

收稿日期:2009-05-26 出版日期:2010-01-26 发布日期:2010-01-27
通讯作者: 霍锐 E-mail:huorui1975@yahoo.com.cn.

Se-scFv-B3 Protecting Neonatal Rat Cardiac againstH₂O₂Triggered Injury

SU Jiaming^1,2, WEI Jingyan¹, SONG Yu³, YANG Lei⁴, HUO Rui¹

1. College of Pharmaceutical Science, Jilin University, Changchun 130021, China;2. College of Life Science, Jilin University, Changchun 130021, China;3. College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China;4.ChinaJapan Union Hospital, Jilin University, Changchun 130033, China

Received:2009-05-26 Online:2010-01-26 Published:2010-01-27
Contact: HUO Rui E-mail:huorui1975@yahoo.com.cn.

摘要/Abstract

摘要：

用H₂O₂损伤大鼠乳鼠的心肌细胞建立氧化应激损伤模型, 考察谷胱甘肽过氧化物酶模拟物Se-scFv-B3对H₂O₂诱导的大鼠乳鼠心肌细胞氧化损伤的影响. 结果表明, Se-scFv-B3能部分增加心肌细胞存活率, 减少细胞凋亡, 恢复线粒体膜电位, 下调Caspase-3活力并降低细胞内活性氧的含量. 表明Se-scFv-B3可以保护心肌细胞抵制H₂O₂诱导的氧化应激损伤.

关键词: 氧化应激；凋亡； Se-scFv-B3；心肌细胞；过氧化氢

Abstract:

To investigate the antioxidant effect of Se-scFv-B3, a model system of H₂O₂induced rat cardiac myocyte damage was established. Se-scFv-B3 was demonstrated to protect cardiac myocytes against H₂O₂induced damage by the increase of cell viability, the decline of cell apoptosis, the inhibition of mitochondrial membrane potential (Δψ_m) collapse, the decrease of Caspase-3 activity and intracellular H₂O₂ after treatment of rat cardiac myocytes by Se-scFv-B3.

Key words: oxidative stress, apoptosis, Se-scFv-B3, cardiac myocytes, hydrogen peroxide

中图分类号:

R392.11

苏家明, 魏景艳, 宋宇, 杨蕾, 霍锐. Se-scFv-B3对过氧化氢诱导的大鼠乳鼠心肌细胞损伤的保护作用[J]. J4, 2010, 48(1): 146-151.

SU Jia-Meng, WEI Jing-Yan, SONG Yu, YANG Lei, HE Dui. Se-scFv-B3 Protecting Neonatal Rat Cardiac againstH₂O₂Triggered Injury[J]. J4, 2010, 48(1): 146-151.