大孔吸附树脂法提取急性子总皂苷的方法

J4 ›› 2011, Vol. 49 ›› Issue (05): 949-953.

大孔吸附树脂法提取急性子总皂苷的方法

李绪文¹, 孟磊¹, 李清民¹, 佟亚楠¹, 金永日¹, 陈伟²

1. 吉林大学化学学院, 长春 130012； 2. 吉林大学中日联谊医院, 长春 130031

收稿日期:2011-07-06 出版日期:2011-09-26 发布日期:2011-09-27
通讯作者: 陈伟 E-mail:chenwei1999@jmgmail.co

Method of Total Hosenkosides Extraction from the Seeds ofImpatiens balsamina L.with Macroporous Adsorption Resin

LI Xuwen¹, MENG Lei¹, LI Qingmin¹, TONG Yanan¹, JIN Yongri¹, CHEN Wei²

1. College of Chemistry, Jilin University, Changchun 130012, China;2. China Japan Union Hospital, Jilin University, Changchun 130031, China

Received:2011-07-06 Online:2011-09-26 Published:2011-09-27
Contact: CHEN Wei E-mail:chenwei1999@jmgmail.co

摘要/Abstract

摘要：

以急性子中4种主要皂苷类化合物凤仙萜四醇苷K,A,C,B质量分数之和为考察指标, 通过正交试验考察了药液的质量浓度、 pH值、流速、乙醇洗脱液的体积分数等影响AB8大孔吸附树脂吸附性能的主要因素, 确定了最优吸附条件. 确定最优的提取方法为：将急性子用体积分数为70%的乙醇回流提取, 提取液浓缩至药材质量的6倍即得药液, 调节其pH值约为6.0, 每小时以3倍树脂体积的流速通过AB8大孔吸附树脂柱, 用体积分数为80%的乙醇洗脱, 此时洗脱物中四种凤仙萜四醇苷K,A,C,B质量浓度之和最高.

关键词: 急性子；总皂苷；大孔树脂；正交试验

Abstract:

Four kinds of main hosenkosides named as hosenkosides K,A,C,B were used as the indexes of total hosenkosides extracted from the seeds of Impatiens balsamina L. The effects of the density, pH and the rate of the flow of the solution on the adsorption properties of the resin were investigated through the orthogonal test, establishing the adsorption conditions. The results show that the solutions were combined after filtration and evaporated to the 6 times the material weight in vacuum. The solution at pH 6.0 was passed through the AB8 macroporous resin at a flow rate of 3 volumes of the resin in 1 h, then eluted by 80% ethanol. This way could collect the total hosenkosides, in which the content of the sum of four hosenkosides K,A,C,B was in the highest point.

Key words: Impatiens balsamina L.； total hosenkoside, macroporous resin, orthogonal test

中图分类号:

O652

李绪文, 孟磊, 李清民, 佟亚楠, 金永日, 陈伟. 大孔吸附树脂法提取急性子总皂苷的方法[J]. J4, 2011, 49(05): 949-953.

LI Xu-Wen, MENG Lei, LI Qing-Min, TONG E-Nan, JIN Yong-Ri, CHEN Wei. Method of Total Hosenkosides Extraction from the Seeds ofImpatiens balsamina L.with Macroporous Adsorption Resin[J]. J4, 2011, 49(05): 949-953.