冷冻-真空吸附法在介孔材料中固定木瓜蛋白酶

冷冻-真空吸附法在介孔材料中固定木瓜蛋白酶

高波^1,2, 朱广山¹, 付学奇², 魏玉红¹, 徐智涵¹, 裘式纶¹

(1. 吉林大学无机合成与制备化学国家重点实验室, 长春 130012； 2. 吉林大学生命科学学院, 长春 130012)

收稿日期:2005-08-03 修回日期:1900-01-01 出版日期:2005-11-26 发布日期:2005-11-26
通讯作者: 裘式纶

Immobilization of Papain in Mesoporous Material via Freezing-vacuum Adsorption Method

GAO ^1,2, ZHU Guang-shan¹, FU Xue-qi², WEI Yu-hong¹, XU Zhi-han¹, QIU Shi-lun¹

(1. State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, Jilin University,Changchun 130012, China; 2. College of Life Science, Jilin University, Changchun 130012, China)

Received:2005-08-03 Revised:1900-01-01 Online:2005-11-26 Published:2005-11-26
Contact: QIU Shi-lun

摘要/Abstract

摘要： 首次采用冷冻-真空吸附法在介孔材料SBA-15中固定了木瓜蛋白酶. 与普通的浸渍方法相比, 此法不仅可以制备高组装量的固定化酶, 每克载体固定量可达450 mg, 同时酶的活性仍然保持, 并得到较好的固定化酶性质(如高稳定性), 在90 ℃保温30 min仍保留70%的活力.

关键词: 冷冻-真空吸附法, 木瓜蛋白酶, 固定化酶

Abstract: Freezing-vacuum adsorption was employed in the immobilization of enzyme in mesoporous material SBA-15. The results indicate that this method can guarantee a large amount of loading about 450 mg/g support, which is incomparable for other means. As long as vacuum degree is properly controlled, the enzymatic activity can be retained. This method provides a new solution for the immobilization of enzyme.

Key words: freezing-vacuum adsorption, papain, immobilization of enzyme

中图分类号:

Q814.2

高波, 朱广山, 付学奇, 魏玉红, 徐智涵, 裘式纶. 冷冻-真空吸附法在介孔材料中固定木瓜蛋白酶[J]. J4, 2005, 43(06): 858-862.

GAO , ZHU Guang-shan, FU Xue-qi, WEI Yu-hong, XU Zhi-han, QIU Shi-lun. Immobilization of Papain in Mesoporous Material via Freezing-vacuum Adsorption Method[J]. J4, 2005, 43(06): 858-862.

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