无花果蛋白酶与木瓜蛋白酶酶学性质的比较

无花果蛋白酶与木瓜蛋白酶酶学性质的比较

曲和之, 黄露, 张国华, 柴智，张雷, 郝东云, 王晓平

吉林大学分子酶学工程教育部重点实验室, 长春 130021

收稿日期:2008-03-14 修回日期:1900-01-01 出版日期:2008-11-26 发布日期:2008-11-26
通讯作者: 王晓平

The Comparison of Enzymatic Characteristics of Ficin and Papain

QU Hezhi, HUANG Lu, ZHANG Guohua, CHAI Zhi,ZHANG Lei, HAO Dongyun, WANG Xiaoping

Key Laboratory of Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun 130021, China

Received:2008-03-14 Revised:1900-01-01 Online:2008-11-26 Published:2008-11-26
Contact: WANG Xiaoping

摘要/Abstract

摘要： 比较了无花果蛋白酶和木瓜蛋白酶的部分酶学性质, 二者均为巯基蛋白酶类, 且水解底物相似. 实验结果表明, 无花果蛋白酶比木瓜蛋白酶的适用性更广, 两者在反应温度范围上各有优点, 无花果蛋白酶的适宜pH值范围大于木瓜蛋白酶的适宜pH值范围; 在对底物的亲合性上, 无花果蛋白酶明显高于木瓜蛋白酶.

关键词: 无花果蛋白酶, 木瓜蛋白酶, 酶学性质比较

Abstract: Ficin and papain are both cysteine proteinase. Comparison of the primer structures suggests the similarities between the active site portions of papain and ficin. In the present study the properties of ficin were analyzed to further know about the enzyme and to extend the comparison of it with papain. Being different from papain, ficin displays a resistance to lower temperatures, a wider range of pH values, and better binding of substrate. The result suggests that ficin is suitable for application.

Key words: ficin, papain, comparison of enzymatic characteristics

中图分类号:

Q554

曲和之, 黄露, 张国华, 柴智，张雷, 郝东云, 王晓平. 无花果蛋白酶与木瓜蛋白酶酶学性质的比较[J]. J4, 2008, 46(06): 1217-1220.

QU Hezhi, HUANG Lu, ZHANG Guohua, CHAI Zhi,ZHANG Lei, HAO Dongyun, WANG Xiaoping. The Comparison of Enzymatic Characteristics of Ficin and Papain[J]. J4, 2008, 46(06): 1217-1220.

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