一种简便高效的古代动物毛皮DNA提取方法

J4 ›› 2010, Vol. 07 ›› Issue (4): 689-693.

一种简便高效的古代动物毛皮DNA提取方法

蔡大伟¹, 于慧鑫², 赵欣¹, 韩璐², 周慧^1,2

1. 吉林大学边疆考古研究中心, 长春 130012|2. 吉林大学生命科学学院, 长春 130021

收稿日期:2009-09-03 出版日期:2010-07-26 发布日期:2011-06-14
通讯作者: 周慧 E-mail:zhouhui@mail.jlu.edu.cn

A Simple and Efficient Method for DNA Extraction fromAncient Animal Dried Skin

CAI Dawei¹, YU Huixin², ZHAO Xin¹, HAN Lu², ZHOU Hui^1,2

1. Research Center for Chinese Frontier Archaeology, Jilin University, Changchun 130012, China;2. College of Life Science, Jilin University, Changchun 130021, China

Received:2009-09-03 Online:2010-07-26 Published:2011-06-14
Contact: ZHOU Hui E-mail:zhouhui@mail.jlu.edu.cn

摘要/Abstract

摘要：

采用试剂盒QIAamp-DNA Mini Kit从距今4 000年前新疆小河墓地出土的黄牛毛皮中提取出古DNA, 并对黄牛线粒体D-loop区部分片段进行了PCR扩增和序列测定. 结果表明: 所提取的古DNA真实可信; 且该方法简便、高效, 适用于古代动物毛皮DNA的提取.

关键词: 动物毛皮；古DNA； DNA抽提, 线粒体D-loop

Abstract:

DNA was successfully extracted from dried cattle skin which was recovered from Xiaohe cemetery, Xinjiang and dated back to 4 000 years ago via QIAamp-DNA Mini Kit. PCR amplification and sequence of mitochondrial D-loop fragment were carried out to evaluate the effect of this method. The results show that the extracted DNA was authentic and reliable.Therefore, this method is simple and efficient to use and is suitable for DNA extraction from ancient dried animal skin.

Key words: dried animal skin, ancient DNA, DNA extraction, mitochondrial D-loop

中图分类号:

Q751

蔡大伟, 于慧鑫, 赵欣, 韩璐, 周慧. 一种简便高效的古代动物毛皮DNA提取方法[J]. J4, 2010, 07(4): 689-693.

CA Da-Wei, XU Hui-Xin, DIAO Xin, HAN Lu, ZHOU Hui. A Simple and Efficient Method for DNA Extraction fromAncient Animal Dried Skin[J]. J4, 2010, 07(4): 689-693.