用气相色谱法研究逍遥丸（水丸）的指纹图谱及其分层聚类分析

J4 ›› 2010, Vol. 07 ›› Issue (4): 683-688.

用气相色谱法研究逍遥丸（水丸）的指纹图谱及其分层聚类分析

梁悦, 魏世刚, 茹鑫, 于爱民, 师宇华

吉林大学化学学院, 长春 130012

收稿日期:2009-09-25 出版日期:2010-07-26 发布日期:2011-06-14
通讯作者: 师宇华 E-mail:shiyh@jlu.edu.cn

Fingerprints with Hierarchical Cluster Analysis forEase Pill by Gas Chromatography

LIANG Yue, WEI Shigang, RU Xin, YU Aimin, SHI Yuhua

College of Chemistry, Jilin University, Changchun 130012, China

Received:2009-09-25 Online:2010-07-26 Published:2011-06-14
Contact: SHI Yuhua E-mail:shiyh@jlu.edu.cn

摘要/Abstract

摘要：

采用水蒸汽蒸馏法提取市售10个厂家逍遥丸（水丸）中的挥发性成分, 并通过气相色谱（GC）进行了分析, 建立了GC指纹图谱, 并用分层聚类分析法对其GC指纹图谱进行聚类分析, 得到了分层聚类分析结果. 实验结果表明：提取的最优条件为提取11 h, 固液比为1 ∶15, 浸泡时间10 h, 选用正十八烷作为内标参照物较适宜. 聚类分析结果表明：不同厂家的逍遥丸色谱中共有峰面积存在差异, 制药过程中的规范和标准有待提高.

关键词: 指纹图谱；气相色谱；分层聚类分析；挥发性成分；逍遥丸

Abstract:

The volatile components in Ease Pill from 10 manufacturers were extracted by hydrodistillation and detected by gas chromatography (GC).GC fingerprints were established and analyzed by the method of hierarchical cluster analysis, by which the discrimination of the samples from different manufacturers was achieved. The optimized extraction parameters were distilling 11 h, a ratio of drug to water of 1 ∶〖KG-*3〗15, soaking 10 h and n octadecane was chosen as the internal standard substance. The results of hierarchical cluster analysis show that there are discriminations among gas chromatography common peaks areas of Ease Pill from different manufacturers, which indicates the standard in medicine production should be improved.

Key words: fingerprint, gas chromatography, hierarchical cluster analysis, volatile components, Ease Pill

中图分类号:

O652.6

梁悦, 魏世刚, 茹鑫, 于爱民, 师宇华. 用气相色谱法研究逍遥丸（水丸）的指纹图谱及其分层聚类分析[J]. J4, 2010, 07(4): 683-688.

LIANG Yue, WEI Shi-Gang, RU Xin, XU Ai-Min, SHI Yu-Hua. Fingerprints with Hierarchical Cluster Analysis forEase Pill by Gas Chromatography[J]. J4, 2010, 07(4): 683-688.