吉林大学学报(理学版)

• 生命科学 • 上一篇    下一篇

树脂对楸灵素吸附与洗脱特性的影响

冯婷婷, 苗辰飞, 于湘莉, 苗志奇   

  1. 上海交通大学 农业与生物学院, 上海 200240
  • 收稿日期:2017-04-01 出版日期:2018-01-26 发布日期:2018-01-24
  • 通讯作者: 苗志奇 E-mail:zqmiao@stju.edu.cn

Effect of Resins on Characteristics of Adsorption and Desorption of Catalitaxol

FENG Tingting, MIAO Chenfei, YU Xiangli, MIAO Zhiqi   

  1. College of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China
  • Received:2017-04-01 Online:2018-01-26 Published:2018-01-24
  • Contact: MIAO Zhiqi E-mail:zqmiao@stju.edu.cn

摘要: 以含有楸灵素的楸树内生真菌FSN002发酵液为原料, 采用静态实验法考察树脂对楸灵素的吸附过程, 建立树脂吸附动力学模型, 并通过模型分析定量研究各种树脂对楸灵素吸附与洗脱特性的影响. 结果表明, 树脂对楸灵素的吸附过程符合一级动力学模型, 其中反相树脂对楸灵素的最大吸附能力平均为39.39 mg/mL, 是离子交换树脂的1.38倍; 反相树脂吸附的楸灵素不易洗脱, 平均洗脱收率为12.2%, 离子交换树脂的平均洗脱收率为46.6%, 其中阴离子交换树脂D201的楸灵素洗脱收率最高达83.0%, 吸附速率较快, 综合性能较好.

关键词: 吸附, 洗脱, 楸灵素, 大孔树脂

Abstract: Using the fermentation broth of the endophytic fungus FSN002 as material, we studied the adsorption process of resins in static test, and established dynamic model of adsorption of resins. The effect of varieties of resins on characteristics of adsorption and desorption were investigated through
the model analysis. The results show that the adsorption of resins to catalitaxol fits firstorder dynamic model, and the average maximum adsorption capacity of reversedphase resins is 3939 mg/mL, 138 times of ionexchange resins.  However, it is difficult to elute the catalitaxol after being adsorbed by the reversedphase resins, and the average elution rate is 122%. While the average elution rate of ion exchange resins is 466%,
and the elution rate of catalitaxol under the anionexchange resin D201 is the highest, which can reach 830%. The adsorption rate is faster, and the comprehensive performance is the best.

Key words: macroporous resin, adsorption, desorption, catalitaxol

中图分类号: 

  • Q503