HPLC法测定木蝴蝶中木蝴蝶苷A和木蝴蝶苷B的质量分数

J4 ›› 2013, Vol. 51 ›› Issue (02): 321-324.

HPLC法测定木蝴蝶中木蝴蝶苷A和木蝴蝶苷B的质量分数

张昌壮^1,2, 张培旭¹, 姚华¹, 杨洁¹, 金永日¹, 李绪文¹, 李清民^3

1. 吉林大学化学学院, 长春 130012； 2. 吉林省烟草产品质量监督检验站, 长春 130062；3. 吉林大学药学院, 长春 130021

收稿日期:2012-07-10 出版日期:2013-03-26 发布日期:2013-03-27
通讯作者: 李清民 E-mail:liqm@jlu.edu.cn

HPLC Determination of Oroxin A and Oroxin B inOroxylum indicum (L.) Vent

ZHANG Changzhuang^1,2, ZHANG Peixu¹, YAO Hua¹, YANG Jie^1,JIN Yongri¹, LI Xuwen¹, LI Qingmin³

1. College of Chemistry, Jilin University, Changchun 130012, China;2. Jinlin Tobacco Quality Supervision and Inspection Station, Changchun 130062, China;3. College of Pharmaceutical Sciences, Jilin University, Changchun 130021, China

Received:2012-07-10 Online:2013-03-26 Published:2013-03-27
Contact: LI Qingmin E-mail:liqm@jlu.edu.cn

摘要/Abstract

摘要：

建立了利用高效液相色谱法(HPLC)同时测定木蝴蝶中木蝴蝶苷A和木蝴蝶苷B的定量分析法: 采用COSMOSILC₁₈MSⅡ（4.6 mm×250 mm, 5 μm）色谱柱, 以V(甲醇)∶V(ρ(磷酸)=0.2%)=4∶6为流动相进行洗脱, 流速1.0 mL/min, 检测波长278 nm, 柱温25.0 ℃. 结果表明: 木蝴蝶苷A的线性范围为0.050~2.0 μg（r²=0.999 9）, 平均回收率为101.14%, 相对标准偏差(RSD)为1.07%；木蝴蝶苷B的线性范围为0.050~2.0 μg（r²=0.999 9）, 平均回收率为100.41%, RSD为2.26

关键词: 木蝴蝶；木蝴蝶苷A；木蝴蝶苷B；高效液相色谱法；质量分数测定

Abstract:

An HPLC method was used for the determination of Oroxin A and Oroxin B in Oroxylum indicum （L.） Vent. A COSMOSILC₁₈MS
Ⅱ（4.6 mm×250 mm, 5 μm） column was adopted. The mobile phase consisted of V(methanol)∶V(φ(phosphoric acid water)=0.2%)=4∶6, at a flow rate of 1.0 mL/min. The wavelength of VWD detector was set at 278 nm and the column temperature was 25.0 ℃. The linear range of Oroxin A was 0.05—2.0 μg （r²=0.999 9）, and the mean recovery was 101.14%（RSD=1.07%, n=6）; the linear range of Oroxin B was 0.050—2.0 μg （r²=0.999 9）, and the mean recovery was 100.41%（RSD=2.26%, n=6）.

Key words: Oroxylum indicum, Oroxin A, Oroxin B, HPLC method, content determination

中图分类号:

R917

张昌壮, 张培旭, 姚华, 杨洁, 金永日, 李绪文, 李清民. HPLC法测定木蝴蝶中木蝴蝶苷A和木蝴蝶苷B的质量分数[J]. J4, 2013, 51(02): 321-324.

ZHANG Chang-Zhuang, ZHANG Pei-Xu, TAO Hua, YANG Ji, JIN Yong-Ri, LI Xu-Wen, LI Qing-Min. HPLC Determination of Oroxin A and Oroxin B inOroxylum indicum (L.) Vent[J]. J4, 2013, 51(02): 321-324.