关键词: 粟酒裂殖酵母, RNA-Seq测序, sgf73基因, 差异表达基因,  , MAPK信号通路

Abstract: In order to study the key genes and key metabolic pathways after the sgf73 gene was knocked out in Schizosaccharomyces pombe,  the wild\|type yeast strains and sgf73Δ  gene-deficient strains were sequenced and bioinformatics analyzed by RNA-Seq sequencing technology, and the GO and KEGG functional enrichment analysis were carried out.  The results show that in the sgf73Δ gene-deficient strains,  there are 1 834 highly expressed genes,  including 6 extremely highly expressed genes,  and 1 714 differentially expressed genes,  of which 934 genes are up-regulated and 780 genes are down-regulated. The  sgf73 gene knockout leads to  abnormal changes in cellular metabolism and transmembrane transport. The  down-regulation of cki1,cki2 and cdc25 genes involved in cycle regulation in the MAPK signaling pathway leads to prolongation of mitotic time in sgf73Δ strain,  the down-regulation of regulatory cytoskeleton rgf2,rho1 and stt4 genes leads to abnormal contraction  of actin ring of  sgf73Δ strain.

Key words: Schizosaccharomyces pombe,  , RNA-Seq sequencing,  , sgf73 gene,  , differentially expressed genes (DEGs),  , MAPK signaling pathway