关键词: 高效液相色谱, 网络药理学, 蒲公英, 抗氧化

Abstract: We studied  the antioxidant active components and mechanism of  dandelion based on  high performance liquid chromatograph (HPLC) method and network pharmacology method.   The effective components of Chinese herbal medicine dandelion were extracted by  organic reagents,  such as petroleum ether,  ethyl acetate,  dichloromethane and n-butanol,  and their antioxidant effects were studied. Multiple online databases of network pharmacology were used to obtain  common targets of dandelion antioxidant construct PPI network,  and conduct GO enrichment analysis and KEGG signal pathway enrichment analysis. The results show that the  extracts of each phase of dandelion have a certain scavenging ability to hydroxy radical (.OH),  superoxide anion radical (O^2-.) and 1,1-diphenyl-2-picrylhydrazyl radical (DPPH.),  the ethyl acetate phase extract of dandelion and the dichloromethane phase extract of dandelion have better scavenging effects,  and the main antioxidant active components are isorhamnetin and oleanolic acid. There are 137 dandelion antioxidant targets screened from the online database. The common targets are mainly concentrated on membrane rafts,  which are resistant to the response of cells to inorganic substances,  nitrogen compounds,  and nutrient levels oxidation. The common targets are closely related to cancer signal pathway,  NF-kappa B signal pathway,  and PPAR signal pathway. 

Key words: high performance liquid chromatography (HPLC),  , network pharmacology, dandelion,  , antioxidant