异鼠李素-3-O-葡萄糖苷和异鼠李素-3-O-芸香糖苷与玉米醇溶蛋白的结合特性和稳定性

吉林大学学报(理学版) ›› 2025, Vol. 63 ›› Issue (2): 629-0637.

异鼠李素-3-O-葡萄糖苷和异鼠李素-3-O-芸香糖苷与玉米醇溶蛋白的结合特性和稳定性

廉笛，崔晶晶，李媛，杜雨桐，王苏青，王梅姿，李丽

长春师范大学化学学院，长春130032

收稿日期:2024-03-22 出版日期:2025-03-26 发布日期:2025-03-26
通讯作者: 李丽 E-mail: lilchem@163.com

Binding Characteristics and Stability of Zein with Isorhamnetin-3-O-glucoside and Isorhamnetin-3-O-rutinoside

LIAN Di, CUI Jingjing, LI Yuan, DU Yutong, WANG Suqing, WANG Meizi, LI Li

College of Chemistry, Changchun Normal University, Changchun 130032, China

Received:2024-03-22 Online:2025-03-26 Published:2025-03-26

摘要/Abstract

摘要： 利用光谱分析和计算机模方法拟研究异鼠李素-3-O-葡萄糖苷和异鼠李素-3-O-芸香糖苷两种黄酮类化合物的成分、与玉米醇溶蛋白（Zein）的结合行为以及两种黄酮-Zein体系的稳定性, 并揭示两种黄酮与Zein的最佳结合模式、结合位点及形成复合物体系的稳定性. 实验结果表明, 两种黄酮均通过氢键和范德华力与Zein结合，导致蛋白固有荧光发生静态猝灭，从而改变蛋白二级结构.

关键词: 玉米醇溶蛋白, 异鼠李素-3-O-葡萄糖苷, 异鼠李素-3-O-芸香糖苷, 多光谱分析, 计算机模拟

Abstract: We studied the composition of two flavonoids, isorhamnetin-3-O-glucoside and isorhamnetin-3-O-rutinoside, their binding behavior with Zein, and the stability of two flavonoid-Zein systems by using spectral analysis and computer simulation method, and revealed the optimal binding mode, binding site and the stability of the complex system formed by two flavonoids and Zein. The experimental results show that two flavonoids bind to Zein through hydrogen bond and van der Waals forces, leading to static quenching of intrinsic fluorescence of Zein and altering its secondary structure of Zein.

Key words: Zein, , isorhamnetin-3-O-glucoside, , isorhamnetin-3-O-rutinoside, , multi-spectral analysis, , computer simulation

中图分类号:

O641.3

廉笛, 崔晶晶, 李媛, 杜雨桐, 王苏青, 王梅姿, 李丽. 异鼠李素-3-O-葡萄糖苷和异鼠李素-3-O-芸香糖苷与玉米醇溶蛋白的结合特性和稳定性[J]. 吉林大学学报(理学版), 2025, 63(2): 629-0637.

LIAN Di, CUI Jingjing, LI Yuan, DU Yutong, WANG Suqing, WANG Meizi, LI Li. Binding Characteristics and Stability of Zein with Isorhamnetin-3-O-glucoside and Isorhamnetin-3-O-rutinoside[J]. Journal of Jilin University Science Edition, 2025, 63(2): 629-0637.