Abstract: Freezing-vacuum adsorption was employed in the immobilization of enzyme in mesoporous material SBA-15. The results indicate that this method can guarantee a large amount of loading about 450 mg/g support, which is incomparable for other means. As long as vacuum degree is properly controlled, the enzymatic activity can be retained. This method provides a new solution for the immobilization of enzyme.

Key words: freezing-vacuum adsorption, papain, immobilization of enzyme