Abstract:

The volatile components in Ease Pill from 10 manufacturers were extracted by hydrodistillation and detected by gas chromatography (GC).GC fingerprints were established and analyzed by the method of hierarchical cluster analysis, by which the discrimination of the samples from different manufacturers was achieved. The optimized extraction parameters were distilling 11 h, a ratio of drug to water of 1 ∶〖KG-*3〗15, soaking 10 h  and n octadecane was chosen as the internal standard substance. The results of hierarchical cluster analysis show that there are discriminations among gas chromatography common peaks areas of Ease Pill from different manufacturers, which indicates the standard in medicine production should be improved.

Key words: fingerprint, gas chromatography, hierarchical cluster analysis, volatile components, Ease Pill