一种简便高效的古代动物毛皮DNA提取方法

J4 ›› 2010, Vol. 07 ›› Issue (4): 689-693.

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A Simple and Efficient Method for DNA Extraction fromAncient Animal Dried Skin

CAI Dawei¹, YU Huixin², ZHAO Xin¹, HAN Lu², ZHOU Hui^1,2

1. Research Center for Chinese Frontier Archaeology, Jilin University, Changchun 130012, China;2. College of Life Science, Jilin University, Changchun 130021, China

Received:2009-09-03 Online:2010-07-26 Published:2011-06-14
Contact: ZHOU Hui E-mail:zhouhui@mail.jlu.edu.cn

Abstract

Abstract:

DNA was successfully extracted from dried cattle skin which was recovered from Xiaohe cemetery, Xinjiang and dated back to 4 000 years ago via QIAamp-DNA Mini Kit. PCR amplification and sequence of mitochondrial D-loop fragment were carried out to evaluate the effect of this method. The results show that the extracted DNA was authentic and reliable.Therefore, this method is simple and efficient to use and is suitable for DNA extraction from ancient dried animal skin.

Key words: dried animal skin, ancient DNA, DNA extraction, mitochondrial D-loop

CLC Number:

Q751

CA Da-Wei, XU Hui-Xin, DIAO Xin, HAN Lu, ZHOU Hui. A Simple and Efficient Method for DNA Extraction fromAncient Animal Dried Skin[J].J4, 2010, 07(4): 689-693.

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A Simple and Efficient Method for DNA Extraction fromAncient Animal Dried Skin

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