Abstract:

Using total RNA from the apothecium of Sclerotinia sclerotiorum, a fulllength cDNA library was constructed by SMART(Switch Mechan
ism at 5′end of RNA Transcript) technology. According to the evaluation, the constructed library had a high titer of 4.11×105 and 97.3% recombinant percentage. 1 800 ESTs from the library were sequenced and compared with the sequences in the GenBank database. By the homologous analysis of 844 unigenes, 602 clones appeared to be completely unknown and were likely to represent newly described ge
nes, whereas 242 clones were identified based on matches to sequences in the database. These identified clones were categorized into three categories: 78 in biological process, 97 in molecular function, 67 in cellular component. In  97 molecular functional clones, a SsMADS gene was obtained which shared high identities with MADSbox and related to sexual reproduction of Sclerotinia sclerotiorum.

Key words: Sclerotinia sclerotiorum； apothecium； cDNA library, expressed sequence tag (EST)； SsMADS gene