Abstract:

A method to calculate the metabolic flux of Saccharomyces cerevisiae metabolism was studied under salt stress conditions. The result shows that under salt stress, the contents of trehalose, glycerol, ethanol and lactate were increased by 24.44%, 60.89%, 23.32% and 26.76%, respectively, the contents of citrate, succinate and fumarate were decreased by 82.11%, 50.37% and 53.33%, respectively. The metabolic flux analysis indicates that the metabolic product flux of each branch channel in EMP was raised at the culture condition of the salt stress, in contrast, each metabolic flux of TCA cycle was reduced.

Key words: Saccharomyces cerevisiae, salt stress, metabolic flux analysis