Abstract:

Chromatographic peaks of different concentration of Nigella sativa L.extacts were characterized by HPLC. B16 murine melanoma cells were used as an in vitro cultured model. MTT was used to study the effect of Nigella sativa L.ethanolic extracts on the proliferation of B16 murine me
lanoma cells. Oxidation rate of LDOPA was measured to estimate the effect of Nigella sativa L.ethanolic extracts on tyrosinase activity of B16 murine melanoma cells. NaOH cleavage method was performed to study the effect of Nigella sativa L.ethanolic extracts on the melanogenesis of B16 murine melanoma cells. Statistical analysis to the chromatographic peaks of different concentration of Nigella sativa L.extacts and pharmacodynamic data for correlation analysis. Results show that 50% ethanolic extract of Nigella sativa L.enhances me lanogenesis and growing rate of B16 murine melanoma cells. Componenteffect relationship shows that there is no significant difference in the 6 chromatographic peaks on pharmacodynamic effect. Pharmacodynamic effect is due to the multiplecomonents of Nigella sativa L.

Key words: Nigella sativa L., proliferation, tyrosinase activity, melanogenesis, correlation analysis