Abstract:

The experiment was designed to use D-galactosamine as ligand, and FFSepharose 4B as immobilization phase to synthesize D-galactosamine-FF-Sepharose chromatography stuffing. Soybean agglutinin was isolated and purified with its purity measured; immunogenicity and agglutination activity were determined and then compared with those of American Sigma company standard sample. The result shows the GalN-FF-Sepharose-4B could adsorb 10 mg of SBA per milliliter. After purification, soybean agglutinin molecular weight was
30 000; SDSPAGE determined purity of it was over 98%; the western blot test was positive; 1 mg/mL SBA hemagglutination titer was 1/2¹⁰; its purity, immunogenicity and agglutination activity were the same as those of soybean agglutinin standard sample and consistent with the theoretical values.

Key words: soybean agglutinin, purification, affinity chromatography