Abstract:

Gene phosphotriesterase from archeaon Thermaerobacter subterraneus DSM13965 was cloned and expressed at high levels in E.coli BL21. The recombinant enzyme was purified to homogeneity via ultrasonic, heating and histag in Nisepharose chromatography. With the help of SDSPAGE and Westernblotting, the phosphotriesterase was identified as a homooligomer existed in dimer form. The enzyme was characterized with ethylparaoxon, its optimum temperature and pH were 70 ℃ and 10.0, respectively. The enzyme has a favourable heat resistance.

Key words: Thermaerobacter subterraneus DSM13965, phosphotriesterase, prokaryotic expression, property