Abstract:

The determination of the new compound of 24(R)majoroside R₁ in Panax japonicus C.A. Mey. var. Major (Burk.[KG-*3]) C.Y. Wu et
K. M. Feng was developed by HPLC\|ELSD method. The conditions of chromatography were Aglient Zorbax SBC₁₈ Column (ODS, 4.6 mm×250 mm, 5 μm) was adopted; mobile phase was composed of V(methanol)∶V(water)=58∶42; flow rate was set at 1.0 mL/min; temperature of column was set at 25 ℃; carrier gas pressure was set at 1.94×105 Pa tube temperature of ELSD was set at 85 ℃; impactor was set in closed state. As the results show in the range of 0.4—20.0 μg, the linearity of 24(R)majoroside R1 is good, r=0.999 3(n=6), the average recovery was 98.7%, and RSD=1.28% (n=6).

Key words: 24(R)majoroside R1, high performance liquid chromatography-evaporative light scatteringdatection (HPLCELSD), mass ratio