Abstract: We studied the composition of  two flavonoids,  isorhamnetin-3-O-glucoside and isorhamnetin-3-O-rutinoside, their binding behavior with Zein, and the stability of two  flavonoid-Zein systems by using spectral analysis and  computer simulation method, and  revealed the optimal binding mode, binding site and the stability of the complex system formed by two flavonoids and Zein.  The experimental results show that  two flavonoids bind to Zein through hydrogen bond and van der Waals forces, leading to static quenching of intrinsic fluorescence of Zein and altering its secondary structure of Zein.

Key words: Zein,  , isorhamnetin-3-O-glucoside,  , isorhamnetin-3-O-rutinoside,  , multi-spectral analysis,  , computer simulation