Abstract:

The leaves of Clinopodium chinensis O.Kuntze var.grandiflorum (Maxim.) Hara.were extracted with 85% volume fraction ethanol under refluxing c
onditions, the extracts were separated by column chromatography with adsorption resin, silica gel and ODS respectively to obtain the chemical constituents, whose structures were elucidated by spectral analysis, physical and chemical evidence. Ten compounds were isolated and identified as buddlejasaponin Ⅳb([STHZ]1[STBZ]), buddlejasaponin Ⅳ([STHZ]2[STBZ]), ClinoposaponinⅪ([STHZ]3[STBZ]), didymin([STHZ]4[STBZ]), 3β,16β,23,28-trihydroxyoleana-11,13(18)-dien-3-yl-［β-D-glucopyranosyl-(1→2)］［β-D-glucopyranosyl-(1→3)］-β-D-fucopyranoside(5), quercetin-3-O-β-D-glucopyranosyl-(1→6)-O-β-D-glucopyanoside([STHZ]6[STBZ]), ClinoposaponinⅤ(7), ClinoposaponinⅢ(8), ClinoposaponinⅤb(9) and ClinoposaponinⅢb(10). Except compounds 1, 2, 4 and 5, all the other compounds were isolated from Clinopodium chinensis O.Kuntze var.grandiflorum (Maxim.) Hara. for the first time.

Key words: Clinopodium chinensis O.Kuntze var.grandiflorum (Maxim.) Hara.； chemical constituents； isolation； identification